Unit 2: Cellular Physiology Flashcards

(54 cards)

1
Q

What are the three main components of the cell?

A

-A plasma membrane, nucleus and cytoplasm

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2
Q

What is the purpose of the Plasma Membrane?

A

Oily layer that separates the ICF from the ECF.

-Allows for selective permeability of substances

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3
Q

What constitutes the Nucleus?

A
  • Double membrane layer that has nuclear pores which allow for selective materials in and out of the nucleus
  • Contains DNA for protein synthesis & to hold genetic information
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4
Q

What is mRNA?

A

Delivers the coded message to ribosomes which “read” mRNA templates and translate it into a proper amino acid sequence

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5
Q

What is rRNA?

A

-An essential component of ribosomes.

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6
Q

What is tRNA?

A

-Transfers the appropriate amino acids within the cytoplasm to their designated site in the synthesizing protein.

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7
Q

What is the cytosol?

A

Semi-fluid medium in which many biochemical events occur within. Contains all the macromolecules.

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8
Q

What is the cytoskeleton?

A
  • Protein network that gives the cell its shape, provides internal organization and regulates its various movements.
  • ->Consists of microfilaments, intermediate filaments and microtubules.
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9
Q

What are organelles?

A

Compartments within the cells that carry out specific tasks. There are 6 organelles:
ER, golgi, lysosomes, peroxisomes, mitochondria and vaults.

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10
Q

Components of the RER?

A
  • Studded with ribosomes, so it undergoes protein synthesis.
  • Proteins can be transported within the cell or secreted elsewhere throughout the golgi.
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11
Q

Components of the SER?

A

-Does not contain ribosomes.
Primarily serves as a central packaging and discharge site for molecules to be transported to the ER.
-Sends molecules to transport vesicles where they can be distributed.

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12
Q

What are vesicles?

A

Cargo containers

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13
Q

What is the main purpose of the SER?

A

Lipid Synthesis

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14
Q

What is the SER in muscle cells?

A

The Sarcoplasmic Reticulum which stores calcium.

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15
Q

What is the Golgi complex?

A

The golgi complex is composed of stacks that bind with vesicles, sort and transport molecules appropriately.
–>Raw materials are turned into finished products and sorted accordingly. Each product is marked with a docking marker to ensure it gets sorted correctly.

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16
Q

Define exocytosis?

A

The mechanism of extruding substances to the cell’s exterior.
“Secretion of contents”

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17
Q

Where do secretory vesicles bind to cells?

A

ONLY to the plasma membrane and not into the actual cells internal membrane.

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18
Q

What are v-snares & t-snares?

A

After vesicles shed coating proteins, docking markers are exposed, which can bind with protein markers on the target membrane.

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19
Q

What are lysosomes?

A

Membrane enclosed sacs that contain powerful hydrolytic enzymes, capable of lysing and destroying cells.

  • Form in the Golgi Complex
  • Like a cell’s miniature digestive system
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20
Q

What is Phagocytosis?

A
  • A type of endocytosis
  • In taking of material by the cell by engulfing the foreign matter.
  • ->Most commonly occurs in WBC’s.
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21
Q

What is Pinocytosis?

A

“Cell Drinking”

  • A small droplet of extracellular fluid is internalized.
  • The plasma membrane drips inward, which seals at the surface around the ECF contents.
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22
Q

What is the name of the protein responsible for pinching off Endocytotic vesicles?

23
Q

Explain Receptor- Mediated Endocytosis?

A
  • Intake of material by receptor- protein binding.
  • Membrane sinks and seals at the surface.
  • Highly selective, but allows intake of large molecules from the environment.
24
Q

What are psuedopods?

A
  • “False Feet”
  • Surround the material to be engulfed and trap it within an internalized vesicle.
  • ->Lysosomes then bind with the vesicle and break down the foreign matter.
25
What is the name of Selective Digestion?
-Autophagy
26
What is Tay-Saach's Disease?
-Abnormal accumulation of of complex molecules formed in nerve cells.
27
What are peroxisomes?
- House oxidative enzymes - ->Use oxygen to strip hydrogen - ->help detoxify waste within cells. - Major product is H202.
28
What does catalase do?
-Turns harmful H2O2 into H20 and CO2 from the oxidative enzymes.
29
Mitochondria Recap.
"Powerhouse of the Cell" Produces 90% of the cell's energy -Contains both a smooth outer membrane and a folded inner membrane (cristae), with a fluid matrix. *Cristae contains proteins that turns food into useable energy (metabolism) -->Majority of metabolic processes occur in the matrix!
30
Glycolysis Recap.
- Turns glucose into 2 pyruvate - Happens in the cytosol. - Takes 10 reactions to do so. - ->Produces 2 ATP/ glucose.
31
TCA Recap.
- Pyruvate is converted into 2 Acetyl CoA by the Pyruvate Dehydrogenase complex. - Produces energy rich molecules ATP, NADH (2 ATP) and FADH2 (1 ATP)
32
Electron Transport Chain Recap
High energy electrons are extracted from NADH and FADH2. | --> Move along the assembly line, and produce cumulatively 30 ATP.
33
Oxidative Phosphorylation
-Occurs when oxygen is present. -->Produces 36 ATP. Starts in cytosol and ends in the matrix.
34
Substrate-Level Phosphorylation
- Occurs when no oxygen is present. - ->Produces 2 ATP/ glucose (Less effective) - Can't proceed beyond glycolysis. - Pyruvic acid is turned into lactic acid.
35
What are vaults?
-Shaped like octagonal barrels -Hollow interior Function: MIGHT be used to transport mRNA -Nucleus to cytoplasm transporters of RNA
36
What is Cytosol?
55% of cell volume - ->Gel like fluid - Surrounds organelles
37
What is inclusions?
-Non-permanent storage houses for fat, glycogen and secretory vesicles in the cytosol.
38
What are the 2 houses for Fat storage?
Adipose tissue (fat droplets) and glycogen (liver and muscle)
39
What are Microtubules?
- Hollow tubules composed of protein TUBULIN. - Determine cell shape and function - Important for spindle formation during mitosis! - ->Make up cilia and flagella.
40
What is Mitotic Spindle?
-Assembled from microtubules only during cell division, and are assembled from centrioles.
41
What are Microfilaments?
- Smallest elements of the cytoskeleton - -->Composed of ACTIN. - Vital for contractile systems. - Important in motility, especially for pseudopods "false feet" in amoebas.
42
What are Intermediate Filaments?
- "Middle size" - Form tough, durable fibres. - Resist mechanical forces/ strain.
43
What makes up DNA?
-A deoxyribose sugar, a nucleotide base and a negative phosphate backbone. A-T C-G
44
2 Main Functions of DNA
- amino acid synthesis | - synthesizing polypeptides
45
What is a gene?
- A stretch of DNA that codes for the synthesis of a particular protein - -Found in chromosomes.
46
How many chromosomes do somatic cells contain?
-46 (23 pairs)
47
How many chromosomes do germ cells (gamates) contain?
Only 1 member of each homologous pair (23)
48
What is the function of histone proteins?
-Key role in packaging DNA into chromosomal structure.
49
What is the function of non-histone proteins?
-Gene regulation.
50
What makes up condensed chromatin?
-Histones + DNA + Non-Histones
51
DNA Replication Recap.
-Double stranded DNA unzips by helicase, and complimentary base pairing occurs on these strands. Creates new templates. **Semi Conservative Process** (One old, one new)
52
Transcription/ Translation Recap.
RNA serves as a "go-between" for DNA sequenced in the nucleus and protein synthesis in ribosomes. - RNA is produced in the nucleus, and exits through nuclear pores into the cytosol where ribosomes are present as mRNA! - mRNA carries the blueprint message. - Once on the ribosome, tRNA puts the nucleotides into amino acid chains. - DNA--> RNA--> Protein
53
Mitosis Recap.
-Daughter cells receive an identical, double strand of DNA. *In multi cellular organisms, mitosis is used for growth and development. Includes interphase, metaphase, anaphase, telophase (cytokinesis)
54
Meiosis Recap.
-Cell division of the gonads. -Produces 4 gamates (daughter cells) Each gamate contains a single strand of DNA. -->2 cell divisions in meiosis -Meiosis 1 includes chromosomal crossing over, which leads to genetic variation. -In meiosis 2, the 23 chromosomes line up and and separate at the end of the spindles. -->Sperms and Eggs are haploid.