Unit 1.2 Structure & replication of DNA Flashcards

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1
Q

The structure of DNA

A

Double stranded helix

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2
Q

What does DNA carry

A

The genetic information for making proteins

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3
Q

What are the four bases that make up the genetic code

A

Adenine, cytosine, guanine, & thymine

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4
Q

What is the complementary base pair rule

A

Adenine always pairs with Thymine, Cytosine always pairs with Guanine

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5
Q

What does each molecule of DNA consist of

A

2 strands, where each strand is made up of repeating units called nucleotides

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6
Q

What does each DNA nucleotide consist of

A

Deoxyribose sugar, phosphate & a base

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7
Q

What type of bond forms between the phosphate of one nucleotide & the deoxyribose sugar of the next nucleotide. What does this create

A

Strong chemical bonds that are not easily broken, this creates the sugar-phosphate backbone

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8
Q

How many different types of nucleotides are there and why

A

4 as there are 4 different types of bases

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9
Q

What are the base pairs joined together by

A

Weak hydrogen bonds that are easily broken

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10
Q

What is it called when the 2 strands of DNA run in opposite directions

A

Anti-parallel

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11
Q

Why is DNA unique

A

It is able to direct its own replication & reproduce itself exactly

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12
Q

Why is it important that DNA replication takes place before a cell undergoes mitosis

A

It ensures each new cell has the correct chromosome complement

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13
Q

What are the 7 stages of DNA replication

A

1- DNA double helix unwinds
2- Weak hydrogen bonds between the bases are broken to form 2 template strands
3- To initiate replication, a primer binds to each template strand at the 3’ end
4- DNA polymerase adds DNA nucleotides (using complementary base pairing) to the 3’ end of the new DNA strand that is forming
5- As nucleotides are added, weak hydrogen bonds form between the base pairs
6- Strong chemical bonds form between the nucleotides of the new strand (forming a sugar-phosphate backbone)
7- Newly formed daughter DNA fold back to double helix

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14
Q

What is each DNA molecule known as after replication

A

Semi-conservative

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15
Q

What does semi-conservative mean

A

Each DNA molecule contains one strand from the original parent & one newly synthesised strand

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16
Q

What is the only direction DNA polymerase can add DNA nucleotides

A

They can only be added to the 3’ end

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17
Q

What DNA strand is replicated continuously

A

The leading strand

18
Q

What DNA strand is replicated in fragments

A

The lagging strand

19
Q

What enzyme joins the fragments of the lagging strand together

A

Ligase

20
Q

What are the 5 requirements for DNA replication

A
  • DNA
  • Primers
  • DNA nucleotides
  • Enzymes
  • ATP
21
Q

What chemical group is found at the 5’ end of the DNA strand

A

Phosphate

22
Q

What is required when studying the genome

A

Millions of identical fragments of DNA need to be created

23
Q

How are millions of identical fragments of DNA created

A

Through the process of PCR

24
Q

What does PCR produce

A

Many copies of a specific section of DNA

25
Q

What does in vitro mean

A

In the lab

26
Q

What does PCR use to amplify DNA

A

Complementary primers for specific target sequences

27
Q

What are primers in PCR

A

Short strands of nucleotides which are complementary to specific target sequences at the 2 ends of the region of DNA to be amplified

28
Q

First stage of PCR

A

Melting: DNA is heated (92-98°C) to break the hydrogen bonds, allowing the original DNA strands to be separated

29
Q

Second stage of PCR

A

Annealing: Sample is cooled (50-65°C). Allows complementary primers to bind to target sequences at the start of each strand

30
Q

Third stage of PCR

A

Extension: Sample is heated (70-80°C) for heat-tolerant DNA polymerase to replicate the region of DNA & adds DNA nucleotides to the 3’ end of the new strands

31
Q

How many identical copies of DNA are made after the first cycle of PCR

A

Two

32
Q

What are the 6 requirements for PCR

A

1- Original DNA sample/template
2- A stock of each of the 4 DNA nucleotides
3- DNA primers
4- Heat-tolerant DNA polymerase (enzyme)
5- Buffer solutions
6- A thermal cycler

33
Q

What do buffer solutions do

A

maintain optimum pH of the enzyme

34
Q

What is a thermal cycler

A

An automated reaction vessel

35
Q

What can PCR be useful for

A

Solving crimes, settling paternity disputes & diagnosing genetic disorders

36
Q

What does amplification mean

A

The number of original molecules has doubled

37
Q

What/ whose DNA samples do forensic scientists take to solve crime

A

DNA samples from the crime scene, the suspect(s) and the victim

38
Q

What then happens to the DNA samples once taken (solving crimes)

A

The components of the samples are separated using gel electrophoresis & compared to identify the criminal

39
Q

What is gel electrophoresis

A

A technique used to separate macromolecules such as DNA

40
Q

Describe how gel electrophoresis works

A

DNA samples are placed in wells of a gel.
An electric current is applied to the gel & since DNA molecules are negatively charged they move towards the positive end of the gel.
As the DNA fragments are different sized the smaller particles move further in the gel thus separating out the DNA fragments

41
Q

How much DNA does an individual inherit from each of their parents

A

50%