Unit 1.2 Structure & replication of DNA Flashcards
The structure of DNA
Double stranded helix
What does DNA carry
The genetic information for making proteins
What are the four bases that make up the genetic code
Adenine, cytosine, guanine, & thymine
What is the complementary base pair rule
Adenine always pairs with Thymine, Cytosine always pairs with Guanine
What does each molecule of DNA consist of
2 strands, where each strand is made up of repeating units called nucleotides
What does each DNA nucleotide consist of
Deoxyribose sugar, phosphate & a base
What type of bond forms between the phosphate of one nucleotide & the deoxyribose sugar of the next nucleotide. What does this create
Strong chemical bonds that are not easily broken, this creates the sugar-phosphate backbone
How many different types of nucleotides are there and why
4 as there are 4 different types of bases
What are the base pairs joined together by
Weak hydrogen bonds that are easily broken
What is it called when the 2 strands of DNA run in opposite directions
Anti-parallel
Why is DNA unique
It is able to direct its own replication & reproduce itself exactly
Why is it important that DNA replication takes place before a cell undergoes mitosis
It ensures each new cell has the correct chromosome complement
What are the 7 stages of DNA replication
1- DNA double helix unwinds
2- Weak hydrogen bonds between the bases are broken to form 2 template strands
3- To initiate replication, a primer binds to each template strand at the 3’ end
4- DNA polymerase adds DNA nucleotides (using complementary base pairing) to the 3’ end of the new DNA strand that is forming
5- As nucleotides are added, weak hydrogen bonds form between the base pairs
6- Strong chemical bonds form between the nucleotides of the new strand (forming a sugar-phosphate backbone)
7- Newly formed daughter DNA fold back to double helix
What is each DNA molecule known as after replication
Semi-conservative
What does semi-conservative mean
Each DNA molecule contains one strand from the original parent & one newly synthesised strand
What is the only direction DNA polymerase can add DNA nucleotides
They can only be added to the 3’ end