Unit 1 - Light, TEM, SEM Ad + Disad

Question 1

Light Microscope Advantages

Answer 1

• Gives good detail
• Can observe living cells
• can be used in many areas e.g. Education, The medical sector
• Gives a quick diagnosis

Question 2

Light Microscope Disadvantages

Answer 2

• Poor resolution of 0.2Mm due to long wavelength of light
• Low magnification of up to 1500X
• Poor surface view
• Very thin sections needed for light to pass through

Question 3

TEM Advantages

Answer 3

• Very powerful magnification of 50 000X
• Images are high quality and detailed
• can see the very small organelles
• High resolution due to short wavelength

Question 4

TEM Disadvantages

Answer 4

• Images are black + white
• Only produces 2D images
• You CANNOT use living organisms

Question 5

SEM Advantages

Answer 5

• Produces 3D images
• Can see the surface of cells
• Produces colour images
• 10X better than Light Microscopes

Question 6

SEM Disadvantages

Answer 6

• Risk of being exposed to radiation
• CANNOT see inside of cells
• Low resolution compared to TEM