Unit 1- Lab Techniques

Question 1

Describe affinity chromatography

Answer 1

It is used to separate target proteins from a mixture.

Question 2

Describe gel electrophoresis

Answer 2

Proteins are on a gel matrix and separate as a current is applied. Proteins are separated by charge size and shape.

Question 3

Describe SDS page

Answer 3

Gives all molecules equally negative charge and denatured them. Separated by size only.

Question 4

Describe native gel

Answer 4

Analyse proteins in folded state. Separated by size and charge.

Question 5

What is an isoelectric point?

Answer 5

The pH of a protein at which it has no net charge. Protein will precipitate out of solution.

Question 6

What are immunoassays?

Answer 6

A technique used to detect specific proteins. It can use antigens to detect antibodies.

Question 7

Describe the use of antibodies to detect antigens.

Answer 7

The antibody specific to target antigen is bound to a plate.
Sample is added onto plate.
If antigen is present it will bind to antibody.
Label antibody specific to antigen is added which can produce a colour change.

Question 8

Describe western blotting.

Answer 8

Used after SDS page. Separated proteins are transferred onto a solid medium.