Ultraviolet Spectrometry Flashcards
Describe the wavelength of uv radiation.
They are shorter than the wavelength of visible light
What is the main source of uv radiation?
The sun
What is the basis of uv spectrometry?
The fact that many compounds absorb IV light is used in the analysis of these compounds
What is UV spectrometry used for?
3
To detect the presence of certain functional groups in molecules
In quantitative analysis to measure the concentration of certain organic compounds in solution
In biochemistry labs to measure the amount of a drug metabolite reaching a certain part of the body
Give some examples of certain drug compounds in solution who’s concentrations can be measured through UV spectrometry
Plant pigments and drugs
What is the principle of UV absorption spectrometry?
That absorbance is directly proportional to the concentration of a substance
What are the three processes that occur in UV spectrometry?
A solution of the substance being analysed is places between a source of UV light and a detector
The detector measures the intensity of light reaching it
A UV absorption spectrum is obtained
Where is the solution of the substance being analysed placed?
Between a source of UV light and a detector
What does the detector measure?
The intensity of the light reaching it
What is obtained at the end of uv absorption spectrometry?
A UV absorption spectrum
What does a UV spectrometer measure?
The absorption of UV radiation by the solution over a range of wavelengths
What tends to happen to the absorption of UV light by a compound?
Depending on the compound, maximum absorption tends to occur at a particular wavelengths
The maximum absorption tends to occur at a particular wavelength, what does this enable?
(2)
This helps to identify the compound
Helps to measure the concentration of the compound in solution
What is UV spectrometry commonly used in conjunction with?
High-performance liquid chromatography
What is UV spectrometry used in conjunction with HPLC for?
To detect the presence of compounds such as transition metals, drug metabolites and plant pigments and to measure their concentrations
