U2 - KA1) Field Techniques For Biologists Flashcards

1
Q

What are the hazards associated with fieldwork

A
  • terrain
  • isolation
  • weather conditions
  • contact with harmful organisms
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2
Q
How are the following hazards 
Terrain 
Isolation 
Weather conditions 
Contact with harmful organisms
A

Terrain - refers to type of ground where fieldwork is conducted- this will dictate the safest type of footwear and whether harness or other safety gear is required. Slipping/ tripping are the most common cause of major injury in UK, so hazards associated with uneven ground must be considered.
Weather conditions - even in areas with normal inncous terrain can become hazardous in sever weather. Any fieldwork in the hills of Scotland requires close consideration of the hazards associated with cold, wet, windy whether. The hazards associated with sun exposure or electrical storms must be considered. Electrical storms have low risk but are a very high hazard.
Isolation - if the fieldwork site is a long distance from habitation , medical help or transport links , this can pose a hazard. Moving supplies or equipment will be more difficult and evacuation procedures will have to be considered in the event of injury or illness. Hazards and risks with lone working also need to be considered.

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3
Q
  • What is risk
  • what is a risk assessment
  • what will an appropriate risk assessment include
A
  • risk is the likelihood of harm arising from exposure to any hazard
  • a risk assessment involves identifying and evaluating these hazards and identifying control measures needed to minimise the risk.
  • a suitable risk assessment would include :
    Identifies signifying hazards
    Evaluate the associated risk (how likely)
    Evaluates the severity of hazard (how harmful)
    Identifies appropriate control measure
    Records these safe working practices
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4
Q

Control measures

A
  • appropriate equipment : fieldworkers should ensure any vehicles are serviced and fuelled. They should also carry a first aid kit, with any specialist drugs related to the habitats in which the are working, such as specific insect repellents. Field equipment should be light and portable. Recording sheets are essential, and appropriate containers for specimens. Cameras and binoculars etc should be in working and clean order.
    Clothing /footwear: appropriate clothes may include windproof or waterproof outer garments as well as specialist clothes such as gauntlets for handling venomous species o veils and hoods for swarming insects. Appropriate footwear may include boosts, wellingtons, waders or gaiters in areas which have ticks etc.
    Communication and orientation - essential that fieldworkers are in touch with the outside world, especially if working in isolated areas. Weather forecast should be checked beforehand. Maps, appropriate permits , a compass , fully charged mobiles, walkie talkies , are essential.information on routes, timescale should be left with appropriate authorities and team leaders should carry home contact details for all members of team. A risk asserts should be carried out.
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5
Q

What is sampling

A

Sampling is the gathering of data from a part of the population. Sampling can eliminate the need to measure and record every member of a population as long as the sample selected is representative of the variation of the population being studied

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6
Q

Minimising impact when sampling - explain what this means

A
  • in some studies wild species may be sampled using observation only. This is preferable but in some cases capture may be needed. If this is the case , consideration needs to be given to minimising the numbers needed to be caught and how sampled individuals can be returned to the wild as rapidly as possible and with minimum handling.
  • if sampling is invasive the benefits of the study must outweigh the negative impact of the sampling
  • In particular it is important to consider whether the sampling involved rare species or vulnerable habitats that are protected by legislation.
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7
Q

What are some sampling techniques

A
  • Point count
  • transect
  • remote detection
  • quadrats
  • capture techniques
  • elusive species
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8
Q

Point count

  • describe it
  • where is it often used
  • what equipment is needed
A

: involves the observer recording all individuals seen from a selected stationary location, which can be compared to counts from other locations.

  • often used for determining species abundance /investigating populations and distributions of species such as birds .
  • cameras, binoculars and telescopes are often used with this technique
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9
Q

Transects

  • line transect
  • belt transect
A
  • are sampling lines laid across habitats affected by environmental gradients such as altitude , light intensity or tidal movement ( so used for determining changes in community across an environmental gradient such as shore)
  • a line transect is normally used to study the distribution of a single plant species (narrow focus of sampling), and those individuals touching the line at a station are counted in the sample
  • a belt transect samples a wider zone along a transect and can be used to study a community of plants or sessile animals. This involved the use of quadrats at each station
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10
Q

Quadrats

A
  • use of quadrats ensures that a standard area is being sampled each time a measurement is made : helped to ensure an equal amount of effort is applied at each sample point which helps to reduce bias and increase reliability
  • suitable for slow moving organisms
  • appropriate size of quadrats is determined by how uniformly organisms are distributed In the habitat
  • smaller quadrats more suitable when the organisms are small or very densely packed
  • diversity influences the number of samples that will need to be taken - more samples required for higher diversities
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11
Q

Remote detection

A
  • monitoring is carried out at distance using sensors for example by satellite. This is used for global vegetation surveys or for gathering data in areas that are difficult to access
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12
Q

Capture techniques for mobile species

A

The sampling of mobile species may involve capture techniques , such as nets or traps

  • birds and bats can be caught in mist nets but only under license
  • night flying insects can be sampled us using a light trap
  • small mammals can be caught in longworth trap
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13
Q

Elusive species
direct evidence
indirect evidence

A

Some animals are very difficult to sample through normal observation . Instead, camera trapping can be used to provide direct evidence or scat sampling (counting droppings) can be used to provide indirect evidence.

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14
Q

What is identification

A

Identification is the ability to put a species name to a sample

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15
Q

How can identification of a sample be made (by using what)

A

Identification of a sample can be made by using classification guides, or dichotomous biological keys or by analysis of DNA or protein

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16
Q

Classification guides

- what info do they give

A
  • classification guides usually deal with a related group of species (focus on one class/order of organism from one location) such as the birds from Europe , dragonflies or butterflies
  • provides information that allows separation of similar species such as description of key features , illustrations , habitat preferences, known distributions and seasonal abundance.
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17
Q

More specialist identification information can be found in the form of ______ _____
- how do these provide info

A
  • biological keys
  • these pose a series of questions , often in the form of paired alternative statements which focus only on the characteristics that allow different species to be separated. In a paired statement key , each option leads to another paired statement or to the conclusive identification of a species.
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18
Q

Analysis of DNA or protein

A
  • a powerful technique for the separation of species that are otherwise only subtly different to one another. Allows identification of species when only partial or molecular evidence remains
19
Q

organisms can be classified by _____ and ______

What does taxonomy involve

A
  • taxonomy anf phylogenetics
  • taxonomy involves the identification and naming of organisms and their classification into groups based on shared characteristics
20
Q
  • Classic taxonomy is based on ________.

- if the taxonomic grouping is known for a particular organism, how can it help scientists ?

A
  • morphology
  • if the taxonomic grouping is known for a particular organism , scientists can make predictions and inferences about its biology based on what is known about its relatives
21
Q

Model organism for a taxonomic group

A

model organisms are those that are easily studied or have been well studied

22
Q
  • how can the study of a model organism be applied to other species , and how is this an advantage
A
  • as the members of a taxonomic group are related, the information from the study of a model organism can be applied to other species in that taxonomic group. This is advantageous as those other species may be more difficult to study directly
23
Q

Important model organisms in advancement of biology

A
Escherichia coli - the bacterium 
Arabidopsis Thaliana - the flowering plant  
Caenorhabditis elegans - the nematode 
Drosophila melanogaster - arthropod 
Mice, rats, fish - chordates
24
Q
  • escherichia coli
  • arabidopsis thaliana
  • caenorhabditis elegans
  • drosophilia melanogaster
  • mice , rats , fish
A
  • bacterial domain: most studied in the bacterial domain. Researchers have been able to culture E. coli in labs for years. Originally it was ti study basic bacterial processes, more recently it’s been used in biotechnology. Eg the use of restriction enzymes to genetically modify plasmids and transform cells was pioneered using E. coli.
  • the flowering plant: small rapidly growing plant of the mustard family. It was the first plant to have its entire genome sequenced and its small genome and small number of chromosomes has made it a good model for the study of genomics , proteomics and metabolics.
  • nematode : the roundworm is the model organism for the abundant and diverse group that forms the phylum Nematoda. This species was the first multicellular organism to have its entire genome sequenced. Much understanding of developmental biology , apoptosis and meiosis stems from research on this species. Many species of nematodes are parasitic so research into this species also has the potential for applications in disease control
  • the fruit fly is a model Arthropod (phylum Arthropoda) much of the research carried out using drosophilia has been rested to genetics, mutation and evolution. We share 75% of our genes with the fruit fly and some of their cells have enlarged chromosomes taht are very easy to study unde the light microscope. This species of fruit fly is very easy to keep in the lab, has a short generation time and many different genetic markers that can be easily studied
  • the chordates (phylum Chordata) mice , rats and zebra fish are amongst the commonly used model vertebrates for the study of normal and abnormal physiology. Neurological and
    Toxilogical studies have been used extensively in all three species
25
Q

What is phylogenetics

A
  • phylogenetics is the study of the evolutionary history and relationships among individuals or groups of species.
26
Q

How is phylogenetics changing the traditional classification of many organisms.

A
  • phylogenetics uses heritable traits , such as morphology , DNA sequences and protein structure to make inferences about an organisms evolutionary history and to create a phylogeny or phylogenetic tree( a diagramattic hypothesis of its relationships to other species )
    Thousands of phylogenetic studies have been combined to reveal the overall pattern of evolutionary relationships of the whole tree of life
27
Q
  • evolution can produce closely related species that look very different to one other, and distantly related species that look similar
    What hides this relatedness
A
  • comparison of genetic evidence revealed relatedness that is obscured by this divergent or convergent evolution
28
Q

Divergent evolution

A
  • divergent evolution is the development of differing life forms from a common origin resulting in forms of life with very different phenotypic characteristics even though they are closely related.
29
Q

How does divergent evolution occur

A
  • it occurs when different selection pressures are acting on each lineage. As a result the ancestral characteristics are lost and replaced by differing adaptive characteristics in the different lineages.
  • divergence can lead to the assumption that two species are very distantly related when in fact they are only different because the same structure has become adapted for different functions
30
Q

What is convergent evolution

A
  • convergent evolution is the separate evolution of similar phenotypic adaptations in lineages whose ancestors did not share these adaptions. (Are not related but have similar phenotypes)
31
Q

How does convergent evolution occur

A
  • this occurs when very similar selection pressures are acting on these unrelated lineages.
  • convergence can lead to the assumption that species are closely related through their appearance , when in fact they are only similar because evolutionary processes modified different structures for a similar function
32
Q

Selection pressures

A
  • factors :
33
Q

Classification : the animal kingdom is divided into phyla , what does this include (taxonomic groupings)

A

: - Arthropoda : made up of invertebrates , with jointed legs and a segmented body , typically with paired appendages.

  • Nematoda , made up of round worms , which are very diverse and many of them are parasitic
  • Chordata- made up of vertebrates and others with a dorsal or spinal notochord
34
Q

What are indicator species

A
  • those whose presence , absence or abundance can give information on environmental qualities such as the presence of a pollutant (eg sulfur dioxide in the air or human faeces in the water)
35
Q
  • each species exists in a niche , so their presence / absence can indicate…
A

Can indicate whether their niche requirements are present or not and so may indicate information about abiotic factors such as temp, oxygen , pH, mineral levels or salinity

36
Q
  • Absence / reduced population of a species indicates that the species is ________ to some factor in the environment
  • abundance or increased population indicates it is ________ by conditions

Give examples

A
  • susceptible/vulnerable or intolerant
  • favoured or tolerant
  • an absence of Lichen - indicates that sulphur dioxide pollution is present in the air
  • an abundance of Midge larvae indicates a lack of dissolved oxygen in polluted fresh water
37
Q
  • An abundance of mayflies indicates….

- an abundance of bloodworms but reduced population of mayflies indicates….

A
  • abundance of mayflies indicates un polluted water that is rich dissolved in oxygen
  • abundance of bloodworms(tolerant to low oxygen levels) / reduce population of mayflies indicates the waterbody is polluted with organic waste
38
Q

Monitoring populations : methods of marking

A

Banding : a visible coded metal/plastic loop attached around part of an organism
Tagging : visible coded metal /plastic tag inserted into/onto the organism
Surgical implantation : microchip inserted in organism , read by electronic reader
Painting : a code painted onto the surface of an organism (such as shelled mollusc)
Hair clipping: a distinctive area of fur trimmed on a small mammal

39
Q

Monitoring populations : what is mark and recapture - how is it done

A

Mark and recapture is a method for estimating population size.
- a sample of the population is captured and marked and released. After an interval of time , a second sample is captured. If some of the individuals in the second sample are recaptured then the total population can be calculated according to an equation .

40
Q

Mark and recapture equation

- assumptions of this method

A
N = MC/R 
N= estimated number of animals in the population 
M= number of animals captured and marked on the first visit 
C= number of animals captured on the second visit 
R= number of animals recaptured animals that were marked (from second sample )

assumptions

  • all individuals have an equal chance of capture
  • no immigration or emigration
  • individuals that are marked and released can mix fully and randomly with the total population
41
Q

Animal behaviour can be studied in wild populations using careful observation and measurement. How might animal behaviour be measured ?

A

Using :
Latency : which is the time between a stimulus occurring and the response behaviour being observed. (Eg the time interval between the appearance of a female stickleback and the male starting his zig zag dance)
Frequency: the number of times a behaviour occurs within the observation period (eg the number of zig zags a male performs in his courtship dance)
Duration: the length of time each behaviour occurs during the observation period ( eg how long the zig zag behaviour lasts )

42
Q

What is anthropomorphism -why does it need to be avoided during measuring animal behaviour

A
  • anthropomorphism is the allocation of human characteristics / behaviour / emotion to non human animals.
  • when analysing animal behaviour anthropomorphism should be avoided , as there is the risk that animal behaviour could be misinterpreted or that any conclusions reached could be invalid.
    Eg (it would be an invalid conclusion to assume that a male stickleback is swimming in a zig zag manner because he is confused or forgetful. Only through objective study does it become apparent that the zig zag behaviour is a sign stimulus to the female to encourage her to follow the male to his nest)
43
Q

What is an ethogram

A
  • an ethogram lists specific behaviours to be observed and recorded in the study, (a list or graphical display of the behaviours shown by species in a wild context)
44
Q

Recoding the duration of each of the behaviours in the ethogram , together with the total time of observation allows the proportion of time spent on each behaviour to be calculated in a ______ _______.

A

Time budget