Trichomonas Vaginalis Flashcards
Habitat of trichomonas vaginalis
Genital tract
Male - urethra, seminal vesicles
Female - vagina, urethra
Morphology of trichomonas vaginalis
Pear shaped
Length 7- 23 micro meters
Width - 5-15
Shows jerky or twitchy mobility
5 flagella. - 4 ant and 1 post, supported by costa
Pathogenicity of trichomonas vaginalis
It’s an obligate parasite which causes mild vaginitis with discharge
Mediated by cell to cell adhesion, hemolysis and release of pore forming protiens
Life cycle of trichomonas vaginalis
•It is an obligate parasite
•Trophozoite is the infective stage
•Divided by binary fission
•Baby can get infected during birth
•Incubation period Is 10 days
•Vaginal ph becomes more than 4.5
Laboratory diagnosis for trichomonas vaginalis
1.Microscopic examination:
Wet mount of Vaginal discharge or scraping, examination of saline mount
2. Elisa
3. PCR
The following stains are used for microscopy for trichomonas vaginalis
1.Papanicolaou
2. Geimsa
3. Leishman
4. Acid schiff
The following media are used for trichomonas vaginalis
- Trussel and johnson’s media - consist of proteose, peptone, back, sodium thioglycollate and normal human serum
- Simplified trypticase serum medium
Ph and temp for culture of trichomonas vaginalis
35°c to 37°c
Ph 5.5 to 6
Steps for preventing trichomonas vaginalis
- Avoidance of sexual contact with infected person
- Use of condom
Treatment for trichomonas vaginalis
Metronidazole 250mg x 3 days
Or 2gm x Dose
Tropically, clotrimazole 100mg x 7 days