Transport in plants Flashcards

1
Q

Why do plants need a transport system

A
  • All living things need certain nutrients and water and a way to get rid of waste
  • This could happen by diffusion for cells on the edge of plants, but cells in the centre of plants aren’t close enough to this supply, and so would die if they couldn’t get these things from elsewhere, as their SA:Vol ratio isn’t sufficient
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2
Q

Distribution of tissues in a dicotyledonous plant root

A
  • Xylem in centre in a distinctive star/x shaped bundle
  • Phloem inbetween the arms of the xylem in bundles
  • Surrounded by the endodermis, just outside a layer of meristem cells called the pericycle
  • Epidermis at edge of plant
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3
Q

Distribution of tissues in a dicotyledonous plant stem

A
  • Vascular bundles at edge of the stem.
  • Distinct in non-woody plants
  • Continuous in older stems of woody plants
  • Xylem on inside of vascular bundle
  • Then the cambium - layer of meristem cells to make new xylem and phloem
  • Then the phloem
  • Then some supporting tissues
  • Cortex on outside of vascular bundles
  • Medulla in centre of vascular bundles
  • Epidermis at edge
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4
Q

Distribution of tissues in a dicotyledonous plant leaf

A
  • Vascular bundles in vein in midrib
  • Xylem above phloem
  • Branching veins - may see other vascular bundles
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5
Q

Structure of xylem vessels

A
  • Thick cell walls, impregnated with lignin (waterproofing, strengthening cell walls so no collapse, even when little water)
  • No end walls and contents - large lumen for water to flow through (decayed when cell died due to waterproofing)
  • Lignin in spiral/rings/broken rings pattern to allow vessel to stretch
  • Pits (gaps in lignin) to allow water to pass from one vessel to another/out into the living tissues of the plant
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6
Q

Function related to structure of xylem vessels

A

Carries water and minerals from roots to tips of plants
-dead cells aligned end to end to form a continuous column
-narrow tubes for capillary action and water column doesn’t break easily
-pits to allow transverse movement of water
-spiralling lignin so can grow and stretch and bend
Flow not impeded because:
-no end walls
-no cell contents (e.g. nucleus or cytoplasm)
-lignin thickening so no collapsing

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7
Q

Structure related to function of sieve tube elemens

A

Transport sugars (usually sucrose) in the form of sap

  • Very little cytoplasm and no nucleus - allow sap to flow
  • lined up end to end
  • cross walls at intervals, perforated to allow sap to flow
  • thin walls
  • 5/6 sided
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8
Q

Structure related to function of companion cells

A
  • large nucleus
  • dense cytoplasm
  • small, fitting inbetween sieve tube elements
  • many mitochondria to provide ATP for active processes needed by sieve tube elements (e.g. loading sucrose)
  • many plasmodesmata to allow communication and minerals to flow
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9
Q

Transpiration

A

The loss of water vapour from the aerial parts of a plant due to evaporation via stomata

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10
Q

Transpiration as a consequence of gaseous exchange

A
  • Water leaves the xylem and passes to the mesophyll cells via osmosis
  • evaporates off surface of mesophyll cells into intercellular spaces
  • water potential rises
  • water diffuses out of leaf via open stomata
  • stomata open for gaseous exchange for photosynthesis
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11
Q

Factors effecting transpiration rate (8)

A
  • number of leaves (more SA to lose water from)
  • wind (removes water vapour from around leaf, maintaining a high water vapour potential gradient)
  • temperature (high temperature means faster diffusion, more evaporation so higher water potential in leaf and less water vapour in air)
  • humidity (higher humidity means lower potential gradient)
  • light (photosynthesis, so stomata are open)
  • cuticle (thick cuticle reduces evaporation from surface)
  • number, size and position of stomata (lots of stomata means more water lost; if on lower surface, less sunlight so less exposure)
  • water availability (not much water means plant can’t replace what it has lost. water loss reduced when stomata shut and leaves are shed in winter)
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12
Q

Potometers

A
  • estimate transpiration rate (only an estimate, as actually measuring water uptake, though as 99% is then lost, it is a good measure)
  • water drawn up capillary tube
  • air bubble/dye to measure movement of water
  • capillary scale to get figures
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13
Q

Water uptake from soil

A
  • root hair cells in epidermis increase SA for diffusion
  • they absorb mineral ions from soil by active transport with ATP
  • lowers water potential of cytoplasm, so water diffuses in via osmosis, down a water potential gradient
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14
Q

Movement of water across the root into the xylem

A
  • moves from epidermis to endodermis by either symplast, apoplast or vacuolar pathways
  • when reaches endodermis, apoplast pathway is blocked by casparian strip, made of waxy suberin (water proof)
  • endodermis actively transports minerals into the xylem, lowering the water potential there
  • water moves in via osmosis
  • this creates a water potential gradient across the whole cortex, as there is a low water potential at the endodermis and high water potential at the epidemis, moving water via the symplast pathway
  • water can also move by the apoplast pathway, which joins the symplast pathway at the endodermis because of the casparian strip
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15
Q

Water movement up the xylem

A
  • root pressure (of water being forced into the xylem) pushes water up the first bit of the stem
  • transpiration pull: water being lost from the leaves must be replaced. As water molecules are cohesive, the water is pulled up the plant in a column. This creates tension in the xylem - yay for lignin! Called the cohesion-tension theory, there is a transpiration stream. Relies on a continuous column of water - if broken, water can leave via pits
  • capillary action: as xylem vessels are narrow and water molecules are adhesive, the forces of attraction can pull water up the sides of the vessel
  • pressure gradient: loss of water from top of plant causes low hydrostatic pressure, water moves from high hydrostatic pressure in roots to low hydrostatic pressure in leaves by mass flow
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16
Q

Water from xylem to air

A
  • leaves xylem via pits and enters mesophyll cells via symplast, apoplast or vacuolar pathways, down a water potential gradient
  • water evaporates off the mesophyll cells into intercellular spaces
  • water vapour diffuses into sub-stomatal spaces
  • if water vapour potential is high in the leaves and low in the air, water vapour will diffuse out of leaves, down a water vapour potential gradient, via stomata
17
Q

Xerophyte

A

A plant that is adapted to reduce water loss so that it can survive in very dry conditions

18
Q

Adaptations of xerophyte leaves (9)

A
  • small leaves/needles (reduces SA for water to be lost from)
  • densely packed spongy mesophyll (reduces evaporation into leaf air spaces as less cells are exposed to the air)
  • thick waxy cuticle (reduces water loss through surface)
  • closing stomata when water availability is low (reduces water loss and need to take up water)
  • hairs on leaf surface (trap air close to leaf, saturated with moisture, reducing water vapour potential gradient)
  • rolled up leaves (lower epidermis not exposed to atmosphere, so traps air to be saturated - reducing water vapour potential gradient)
  • only opening stomata at night (less heat, so less water loss)
  • stomata in pits (pits become saturated with water vapour, reducing water vapour potential gradient)
  • low water potential inside leaves (maintaining a high salt concentration, thus reducing evaporation of water and therefore the water vapour potential gradient)
19
Q

Translocation

A

An energy-requiring process transporting assimilates (mainly sucrose) between sources and sinks. Occurs in the phloem tissue

20
Q

Translocation at the sources

A
  • sucrose is actively loaded into the companion cells:
  • hydrogen ions are pumped out, creating a concentration gradient
  • as they diffuse back, they bring sucrose
  • sucrose then diffuses into the sieve tube elements
  • this lowers the water potential in the sieve tube elements, meaning water moves in via osmosis
  • this creates a high hydrostatic pressure
21
Q

Translocation at the sinks

A
  • sucrose is used up in the surrounding cells (either for storage or in metabolic processes)
  • this reduces the concentration of sucrose, creating a concentration gradient
  • sucrose is diffused/actively transported out of the phloem
  • this increases the water potential gradient, so water moves out, creating a low hydrostatic pressure
22
Q

Translocation along the phloem

A
  • water flows from the high hydrostatic pressure at the source to the low hydrostatic pressure at the sink, along a pressure gradient: mass flow
  • can occur up or down a plant (could be different in different tubes)
23
Q

Examples of sources and sinks

A

sources: leaves, roots (releasing stored carbohydrates)
sinks: roots (growing), seeds (when storing carbohydrates)

24
Q

Evidence for translocation by mass flow

A

We know the phloem are used:
-radioactive carbon in phloem (carbon dioxide to sucrose)
-ringing a tree (cutting off phloem) produces a bulge where sugar collects and water flows there
-aphids feed from phloem
We know it requires energy:
-mitochondria in companion cells
-stops when a metabolic poison is applied that inhibits formation of ATP
-rate of flow is high, so energy must be needed to drive the flow

25
Q

Evidence against translocation by mass flow

A
  • not all solutes in the sap move at the same rate
  • sucrose is moved to all parts of the plant at the same rate, rather than travelling quicker to areas with lower concentrations
  • role of the sieve plates is unclear
26
Q

Setting up potometers

A
  • shoot is healthy
  • assemble apparatus and cut shoot under water
  • cut off last 2-3 cm of shoot at an angle
  • ensure no air bubbles in apparatus
  • ensure apparatus is air tight
  • ensure leaves of shoot are dry