Transport across the cytoplasmic membrane Flashcards
what is passive diffusion
the movement of molecules from higher concentration to lower concentration without the use of energy
what is facilitated diffusion
when molecules move from higher concentration to lower concentration through a permease without the use of energy
what examples of molecules can move by passive diffusion
O2, H2O,CO2
What is the saturation effect observed in carrier-mediated transport systems?
Carrier-mediated transport systems have an increase in rate of uptake until fully saturated
describe the three classes of membrane transporters
Uniporters: transport molecules in one way.
Antiporter: transport molecules simultaneously in two different directions.
Symporter: transport molecules simultaneously in the same direction.
what is an example of a symporter
Lac permease in E. coli.(Lacotse and H+ into the cell)
what is an example of antiporter
Sodium-calcium exchanger
what are the three main classes of transport in prokaryotes?
Simple transport(proton motive force)
ABC system
Group translocation
Explain the active transport
this is the process of moving molecules against their concentration gradient
what does ABC stand for?
ATP Binding Cassette
what type of substrate specificity does ABC have
high substrate specificity
Examples of molecules that are moved in the ABC system
sugar. amino acids
what are the components of the ABC system
periplasmic binding protein (sugar or solute binding protein)
integral binding protein (permeases binding protein)
ABC protein that provides ATPase function
gram positives employ what type of substrate binding proteins
lipoproteins
ATP binding cassette is located where for a gram negative bacteria
in the periplasm
ATP binding cassette is located where for a gram positive bacteria
in the cell membrane
briefly explain the ATP binding cassette
a solute diffuses into the periplasm and binds to a solute binding protein. the solute then binds to the integral membrane protein. ABC protein hydrolyses ATP to ADP before the solute is translocated to the cytoplasm.
what does the hydrolysis of ATP provide
energy for the translocation of the solute
what is group translocation
a molecule is chemically altered as they cross the membrane
what is the PTS
phosphotransferase system
PTS uses what as an energy source
PEP (phospho-enol-pyruvate.)
what are the common components of the PTS and where are they located
enzyme i(cytosolic)
HPr(cytosolic)
what are the specific components of the PTS
enzyme iia(cytosolic)
enzyme iiib(cytosolic or membrane)
enzyme iiic(membrane)
briefly explain the PTS
PEP donates its phosphate group to enzyme i to become EI-P which then donates the phosphate group to HPr which then becomes HPr-P then donates its phosphate group to EIIA to become EIIA-P then to EIIB to become EIIB-P then finally to glucose which becomes glucose-6-phosphate
what is the purpose of phosphorylation
to prevent it from diffusing out of the cell
what is the function of the cell wall in a bacteria cell
to prevent osmosis lysis
to prevent toxic substances
helps to evade host immune system
what are the two layers in a gram negative bacteria cell wall
lipopolysaccharide
peptidoglycan
what are the components of peptidoglycan
N-acetylglucosamine and N-acetylmuramic
Amino groups
lysine or diaminopimelic acid(DAP)
form glycan tetrapeptide