Transport 1

Question 1

Name the three types of coated vesicles

Answer 1

Clathirin
COP I
COP II

Question 2

Clathirin

Answer 2

Mediates transport from Golgi & Plasma Membrane

Composed of 3 large and 3 small polypeptide chains in a Triskelion—> Basket-like with secondary layer of adaptor proteins

Question 3

COP I

Answer 3

Mediates transport from Golgi

Question 4

COP II

Answer 4

Mediates transport from ER

Question 5

___ plays a major role in coat assembly, vesicle formation and protein trafficking. Specifically, ___ and ____/

Answer 5

Phosphoinositides, phosphorylation and dephosphorylation

Question 6

Describe the process of Vesicle Pinching

Answer 6

Dynamin wraps around a clathrin bud and squeezes with help of hydrolyzed GDP

Question 7

What are the two proteins involved vesicle targeting?

Answer 7

RAB and SNARE

Question 8

RAB

Answer 8

Directs vesicle to specific spots on target membranes

Question 9

SNARE

Answer 9

Catalyze fusion of vesicles with membranes via twisting mechanism with t-snare and v-snares.

Question 10

What is the active form of RAB?

Answer 10

Membrane bound

GTP bound form with Rab-GEF

Question 11

RAB1 found

Answer 11

in ER and Golgi Complexes

Question 12

RAB3A found in

Answer 12

Found in synaptic vesicles, secretory vesicles

Question 13

Rab5 found in

Answer 13

Found in early endosomes, plasma membrane, clathrin-coated vesicles

Question 14

How does SNARES untangle after vesicle fusion?

Answer 14

NSF with the expenditure of ATP causes this untangling

Question 15

____ and ___ are the quality control chaperones involved in vesicle transport from ER

Answer 15

BIP and Calnexin

Question 16

What is the signal sequence that recognizes vesicles to come back when near a ER membrane?

Answer 16

KKXX

Question 17

What is the signal sequence that recognizes vesicles to come back when in the cytosol?

Answer 17

KDEL