Translocation Flashcards

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1
Q

What is it

A

The transport of ions and organic/inorganic molecules

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2
Q

Where does translocation occur in flowering plants

A

The phloem

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3
Q

What’s the phloem made up of

A

Living (respiring) cells

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4
Q

E.gs of what the phloem transports

A
Amino acids
Sucrose
Glucose
Potassium
Chloride
Magnesium
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5
Q

Where does photosynthesis produce plants

A

In areas called sources

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6
Q

Why can’t the transport in the phloem be down to diffusion

A

It’s too fast

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7
Q

What is mass transport

A

Bulk transport of a substance through a given channel in a given time

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8
Q

Explain the mass flow theory (1-5)

A
  1. Sucrose made my cells with chloroplasts
  2. Moves into companion cells by facilitated diffusion
  3. Companion cells actively pump hydrogen ions into spaces between cell walls (active transport)
  4. Hydrogen ions move into sieve tube elements by facilitated diffusion
  5. Sucrose is transported with hydrogen - cotransport via cotransport proteins
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9
Q

Explain the mass flow theory (6-11)

A
  1. Build up of sucrose in sieve tubes lowers their water potential
  2. Water from xylem moves into sieve tubes by osmosis creating a high hydrostatic pressure
  3. Conc drops as sucrose is used by respiring plant cells
  4. Sucrose is actively transported into plant cells from sieve tubes due to low sucrose levels
  5. Water enters plant cells from sieve tubes by osmosis due to their lowered water potential
  6. Hydrostatic pressure falls in sieve tubes
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10
Q

What causes a hydrostatic gradient in mass flow theory

What does this help occur

A

The difference in hydrostatic pressure in the sieve tubes at the sinks (low) and sources (high)

Means mass transport in phloem can occur

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11
Q

What 4 gas exchange process are involved in the mass flow theory

A

Facilitated diffusion
Active transport
Co-transport
Osmosis

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12
Q

How does sucrose get to phloem

A

Via facilitated diffusion from palisade cells through the spongy mesophyll -> phloem

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13
Q

What’s the gaps in between cells called

A

Plasmodesmata

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14
Q

Evidence for mass flow theory

A

Cell sap is released when phloem is cut due to sieve tubes’ hydrostatic pressure
Sucrose concentration greater in leaves than roots
Downward flow of sucrose during light times of day (photosynthesis)
Increases sucrose in leaves follow by increased sucrose in phloem shortly after
Poisons that hinder respiration/lack of oxygen reduce translocation - no active transport
Companion cells contain mitochondria/ATP

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15
Q

Evidence against mass flow theory

A

Presence of sieve plates
Not all molecules move at same speed - should in mass flow
Sucrose is delivered to all areas at same rate - not delivered quicker to areas that need it most (conc gradients)

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16
Q

How do aphids help investigate phloem

A

Aphids (green fly) feed on sucrose in phloem using a proboscis
They insert the proboscis into the phloem
The aphid is anaesthetised and it’s head cut off
Scientist can then collect contents from phloem using proboscis

17
Q

2 other ways of investigating phloem

A

Radioactive tracers

Ringing

18
Q

How do radioactive tracers work

A

Plant supplied with radioactive carbon in CO2 (14C)
This carbon is used to produce sugars in the plant
These can be traced as they travel

19
Q

What’s the evidence of the presence of phloem/transport of sugars from radioactive tracers

A

Areas of radioactivity appearing ‘foggy’ on the photographic film

20
Q

What is placed on photographic film in the dark to test for phloem

A

Cross sections of stems that are removed

21
Q

What can test for radioactivity of sap (aphids)

A

Aphids feeding at different locations on a plant

22
Q

What happens in ringing

A

Cylinders of bark + some tissue from beneath are removed from plant stem
Phloem is removed, xylem is left intact
Contents of phloem, above + below the ring can be sampled and analysed using a similar technique to the aphid

23
Q

Where do sugars build up when investigating phloem

A

Above the ring in ringing as they can’t be transported any further