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Translation > Translation in PKs > Flashcards

Translation in PKs Flashcards

1
Q

Initiation is GTP independent or Dependent?`

A

Dependent

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2
Q

Association of Small and Large sub0unit of Ribosome happens at?

A

Ribosome Binding Site

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3
Q

At RBS _____ sequence present.

A

Shine Dalgaro sequence

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4
Q

Initiating Codin

A

AUG
GUG
UUG

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5
Q

Initiating amino acid in E.coli

A

N-Formyl Methionine

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6
Q

Initiating amino acid in Archae Bacteria

A

Methionine

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7
Q

Shine Dalgaro Sequence

A

Short ntd. seq.
7 ntds.: 5’ AGGAGGU 3’
Purine rich
Slight sequece variation

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8
Q

Shine Dalgaro Sequence Located at?

A

3-10 ntds. upstream of the Start codon.

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8
Q

Complementary sequence of Shine Dalgar is present at?

A

@ 3’ end of 16S rRNA

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9
Q

Translational Coupling

A

It refers to the mechanism by which the translation of one mRNA molecule is linked to the translation of another, often leading to coordinated expression of genes that may be functionally related.

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10
Q

N-Formyl Methionine and Methionine

A

Initiation Codon (IC) is same for both

But tRNA is different

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11
Q

tRNA for N-Formyl Methionine

A

tRNA^fmet

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12
Q

tRNA for Methionine

A

tRNA^met

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13
Q

Enzyme for N-Formyl Methionine

A

Met tRNA Synthetase: Amino acylates bot tRNA with Met

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14
Q

What promotes active translation?

A

High Complementarity b/w RBS & rRNA

Proper spacing b/w RBS and Start Codon

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15
Q

Amino acid binds with tRNA with?

A

Ester bond

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16
Q

If an ORF lacks RBS, will they be translated. Yes or No?

A

Yes

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17
Q

Transformylase

A

Transfer formy group from

N^10 Formyl Tetrahydrofolate to amino group of Met.

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18
Q

which is more selective?

Transformylase or Met-tRNA synthetase

A

Transformylase

19
Q

Functions of added formyl?

A

Prevent fmet from entering the interior position in polypeptide.

allow it to bind at initiation site

20
Q

Extra GC Sequence and single CA sequence is present on?

A

Anticodon arm of tRNAi^met

21
Q

is it necessary that the first peotide will always be Fmet, as tRNA^fmet incorporated first?

A

No

22
Q

which enzyme removes the formyl group f amino terminus from the polypeptide?

A

Deformylase

23
Q

Aminopeptidases

A

often removes the amino-terminal methionine and additional to-three amino acids

24
Q

Initiation Factors

A

IF1
IF2
IF3

25
Q

Polypeptide synthesized from nitochondria and chloroplast ribosomes begin with ____________?

A

N-Formylmethionine.

This suggests that mitochondria and chloroplast are originated from the bacterial ancestors.

26
Q

Binary complex of IF

A

IF2 + GTP

27
Q

Ternary Complex of IF

A

IF2 + GTP + tRNAi^fmet

28
Q

Steps?

A

30S + IF1 + IF3
fmet-tRNA + IF2 + GTP

29
Q

what inhibits 30S & 50S SUBUNITS FROM COMBINING prematurely?

A

IF3

30
Q

IF3 promotes?

A

mRNA binding to the 30S subunit, and occupy that region which will become the E-Site

31
Q

what does IF1 do?

A

it binds to the ‘A’ site, and prevent binding of tRNA during initiation

32
Q

mRNA is bound by____?

A

both GTP bound IF2 and fmet-tRNA^fmet

33
Q

what part of tRNA pairs with mRNA?

A

Anticodon

34
Q

Elongation Factors?

A
  1. EF-Tu: GTP Binding protein &
    GTPase
  2. EF-Ts: Regenerate EF-TI
  3. EF-G: GTP Binding protein &
    GTPase
35
Q

Binary Complex of EF?

A

EF-Tu-GTP

36
Q

Ternary Complex of EF?

A

EF-Tu-GTP+tRNA^aa

37
Q

3 sub phases of elongation?

A
  1. Decoding : By EF-TU
  2. Transpeptidation: By 50S-23S rRNA
  3. Translocation: By EF-G
38
Q

Decoding?

A

when amino acyl tRNA enters at ‘A’ site, performed by EF-Tu

39
Q

Transpeptidation?

A

Amino acid present on the ‘P’ site added to the a.a present on the ‘A’ site.

40
Q

Peptidyl transferase activity

A

when Amino acid present on the ‘P’ site added to the a.a present on the ‘A’ site.

41
Q

Translocation?

A

Movement of Ribosome.

Translocation results into conversion of ‘P’ site into ‘E’ site and ‘A’ site into ‘P’.

And new ‘A’ site will be formed.

42
Q

Which EF act as a GEF?

A

EF-Ts

43
Q

Active form of EF-Tu

A

EF-TU—–GTP

44
Q

Inactive form of EF-Tu

A

EF-TU—–GDP

Translation (4 decks)

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