Translation Flashcards
tRNA
“Translator” reads nucleotide word and interprets it as a protein word
3’ end
Attachment site
Anticodon
Nucleotide triplet that base pairs to a specific mRNA codon
Initiation
Transcription factors attach to DNA and polymerase is bound in the correct orientation
The enzyme then unwinds the 2 DNA strands to being transcription
TATA box
Nucleotide sequence about 25 nucleotides upstream from the starting point
Termination in bacteria
terminator is in DNA; polymerase releases transcript
Termination in Eukaryotes
RNA polymerase II transcribes a stop sequence
Once the polymerase catch up to each other, it detaches
Phases
Initiation
Elongation
Termination
Binding Sites of Elongation
P-Site: polypeptide
A-Site: amino acid
E-Site: exit
Translocation
Between step 2 and 3 of elongation
tRNA slide over one spot so that the polypeptide chain is back in the middle (p-site) and the empty tRNA can exit
Initiation codon is found
Within the mRNA