Translation

Question 1

What is the size of eukaryotic ribosome?

Answer 1

80S (60+40)

Question 2

What is the size of prokaryotic ribosome?

Answer 2

70S (50+30) - cleft is where protein synthesis occurs

Question 3

What is tRNA?

Answer 3

Transfer RNA is a small RNA molecule that plays a key role in protein synthesis. tRNA serves as a link (or adaptor) between the mRNA molecule and the growing chain of amino acids that make up a protein.

Question 4

What links with the codon in tRNA?

Answer 4

Has an anti-codon that is complimentary able to H-bond. alignement is antiparallel

Question 5

What is at the end of tRNAs?

Answer 5

CCA

Question 6

What are the diff parts of tRNA?

Answer 6

AA arm - has AA esterified via carbox grp to 2’ or 3’-OH of A terminal in CCA codon.
Anticodon arm
D arm - dihydrouridine (D), contributes to folding.
TyC arm - pseudouridine - unusual C-C bonding btwn base and ribosome. helps folding.

Question 7

What are wobble pairings?

Answer 7

A wobble base pair is a pairing between two nucleotides in RNA molecules that does not follow Watson-Crick base pair rules. Wobble allows tRNAs to recognise more than one codon (3rd codon adds nothing to specificity). eg redundancy of AA