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Micro Lab Exam 2 > Transformation of Bacteria > Flashcards

Transformation of Bacteria Flashcards

1
Q

competent

A

capable of taking up DNA

EX. E. COLI

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2
Q

transformation

A

TAKING UP DNA

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3
Q

purpose and process of transformation

A

EXPOSE TO DNA OF INTEREST AND GET THEM TO TAKE IT UP. THE PURPOSE IS GENETIC ENGINEERING TO PRODUCE VACCINES

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4
Q

purpose of heat shock and calcium salt

A

makes membrane more permeable

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5
Q

purpose of LB agar

A

improves recovery and survival

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6
Q

purpose of plating on media containing the antibiotic ampicillin

A

REDUCES RISK OF CONTAMINATION, SELECTS FOR ONLY TRANSFORMED CELLS

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Micro Lab Exam 2 (14 decks)

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