Transcription & Translation Flashcards
What is transcription
The synthesis of mRNA from a section of DNA
What is the role of RNA polymerase
It moves along the DNA unwinding the double helix and breaking the hydrogen bonds and adds new RNA nucleotides following RNA base pairing rules
What is left after the RNA polymerase
The primary transcript
What are introns
Regions of the transcript that are non-coding
What are exons
The coding regions
What happens during RNA splicing
Introns are cut out and exons are joined together( order of exons unchanged)
After RNA splicing what is the transcript known as
The mature transcript (code for a protein)
What is translation
The synthesis of a polypeptide from mRNA at the ribosome
Where does translation occur
The cytoplasm
First stage of translation
tRNA picks up a specific amino acid and takes it to the ribosome
2nd stage of translation
Codon and & anti codon pair up using complementary base pairing
3rd stage of translation
Peptide bond forms between adjacent amino acids
4th stage of translation
tRNA is released to pick up another amino acid
What happens after the 4th stage of translation
Amino acids joined by peptide bonds
Describe alternative RNA splicing
Different segments of mRNA may be treated as exons and introns. The same primary transcript can produce several mature transcripts