Transcription/Translation Flashcards
After eukaryotic transcription mRNA
is usually processed. What is the
correct sequence of processing events?
RNA transcription - addition of 5’ cap
- addition of
poly-A tail - intron removal - transport from nucleus
translation.
What is the name of the protein/RNA
complex that undertakes translation
within the cell?
Ribosome
In investigations of the lac operon we
can utilise a variety of lactose
analogues. One of these is called IPTG.
What is this particularly used for?
Binding to the Lac repressor to induce the lac operon
Which of the following types of
protein are involved in the regulation of
gene expression?
Repressors and Activators
What is the name of the region within
the promoter of eukaryotic cells that
recruits the transcription initiation
complex?
TATA box
A total RNA extraction is the
first stage in the process of studying
gene expression. What do the two largest bands represent?
28S and 18S Ribosomal RNA bands
Which one of the following
statements about transcription is
correct?
In eukaryotes introns are non-coding sequences that
are usually removed from mRNA molecules.
Adenylate cyclase converts….
ATP to cyclic AMP
Which of these statements about
regulation of the tryptophan operon is
correct?
- The trp genes are turned on in the presence of excess
tryptophan. - The trp genes are turned on in the presence of lactose.
- When levels of tryptophan are low in the cell then the genes encoding for tryptophan biosynthesis enzymes
are turned on. - Binding of tryptophan to the Trp repressor protein
removes it from the operator - When glucose levels are high the cell does not require
tryptophan
When levels of tryptophan are low in the cell then the
genes encoding for tryptophan biosynthesis enzymes
are turned on.
In the Lac operon, the purpose of the
LacY protein is to..
Allow entry of lactose into the cell
