Transcription/Translation Flashcards

1
Q

After eukaryotic transcription mRNA
is usually processed. What is the
correct sequence of processing events?

A

RNA transcription - addition of 5’ cap
- addition of
poly-A tail - intron removal - transport from nucleus
translation.

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2
Q

What is the name of the protein/RNA
complex that undertakes translation
within the cell?

A

Ribosome

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3
Q

In investigations of the lac operon we
can utilise a variety of lactose
analogues. One of these is called IPTG.
What is this particularly used for?

A

Binding to the Lac repressor to induce the lac operon

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4
Q

Which of the following types of
protein are involved in the regulation of
gene expression?

A

Repressors and Activators

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5
Q

What is the name of the region within
the promoter of eukaryotic cells that
recruits the transcription initiation
complex?

A

TATA box

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6
Q

A total RNA extraction is the
first stage in the process of studying
gene expression. What do the two largest bands represent?

A

28S and 18S Ribosomal RNA bands

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7
Q

Which one of the following
statements about transcription is
correct?

A

In eukaryotes introns are non-coding sequences that

are usually removed from mRNA molecules.

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8
Q

Adenylate cyclase converts….

A

ATP to cyclic AMP

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9
Q

Which of these statements about
regulation of the tryptophan operon is
correct?

  • The trp genes are turned on in the presence of excess
    tryptophan.
  • The trp genes are turned on in the presence of lactose.
  • When levels of tryptophan are low in the cell then the genes encoding for tryptophan biosynthesis enzymes
    are turned on.
  • Binding of tryptophan to the Trp repressor protein
    removes it from the operator
  • When glucose levels are high the cell does not require
    tryptophan
A

When levels of tryptophan are low in the cell then the
genes encoding for tryptophan biosynthesis enzymes
are turned on.

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10
Q

In the Lac operon, the purpose of the

LacY protein is to..

A

Allow entry of lactose into the cell

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