Transcription Regulation Flashcards
What are the 2 basic methods of Transcription Activation?
1) TFII/ Pol II recruitment
2) Chromatin modification and remodeling
What are two transcription regulators in Euk.?
Enhancers and Promoter proximal elements(PPEs)
What enzyme is used for Nucleosome remodeling and what does iti do?
DNA Helicase; moves nucleosomes
What enzymes are used for nucleosome modification activities?
acetylases/deacetylases, methylases/demethylases, and kinases`
What is the role of Histone Acetylases(HAT)?
HAT acetylases histones and as a result activates transcription
What is the role of Histone Deacetylases(HDAC)?
HDAC deacetylate histones and as a result repress transcription
What is the result of CpG methylation?
transcription repression
In general, what is the role of Transcription Activators?
TFII/Pol-II recruitment
Histone acetylation/methylation
Nucleosome remodeling
In general, what is the role of Transcription Repressors?
Histone deacetylation/methylation
CpG methylation
What is the role of H3K9 Histone Methyl Transferase?
Heterochromatin spreading
Which two factors bind in order to recruit H3K9 Histone Methyl Transferase(HMT)?
HP1 and H3K9-Me3
What is the role of Inulators(boundry elements)?
To repress influence from neighboring chromatin regions
How do steroid hormones regulate gene expression?
they act on transcription factors (TFs) and cause cell to cell signaling
What is the role of a ChIP assay?
identifies genomic positions of proteins(in vivo)
