Transcription

Question 1

What do siRNA’s do?

Answer 1

inhibit target mRNA (so effects translation) and is part of the RISC complex which degrades RNA

Question 2

What do miRNA’s do?

Answer 2

post translational regulation of gene expression

Question 3

Where is rRNA made?

Answer 3

nucleosome

Question 4

Homeodomain proteins as transcription regulators

Answer 4

Binding motif for DNA domain - HOX genes

Question 5

Zn finger protein and transcription regulation

Answer 5

Cys-His regions that interact with Zn to make loops

bind to the major groove and effect transcription

Question 6

Leucine zipper domain and DNA transcription

Answer 6

Every 7th residue is leucine and dimerizes/coils

Question 7

helix loop helix protein and transcription regulation

Answer 7

binds and regulates

Question 8

Fragile X Mental Retardation is caused by what?

Answer 8

mutation in the FMR1 gene which codes for things important for neuro
mutation is and increase in repeats of a cytosine rich region which are succeptable to methylation (T-G and T is removed causing frameshift mutation) - the more repeats, the worse the disease

Question 9

What do snRNA’s do?

Answer 9

are part of the splicosome complex which splices pre-mRNA into mRNA (splices out the introns)