transcription 2

Question 1

how many classes of activators of transcription is there for RNA polymerase

Answer 1

there is class 1 and class 2 activators along with protein to protein interactions that are sufficient for activation

Question 2

explain the class 1 activator of transcription

Answer 2

binds DNA upstream of -35 and requires alpha CTD interaction

Question 3

explain the class 2 activator of transcription

Answer 3

binds overlapping -35 and requires sigma interaction alpha CTD dispensable

Question 4

do promoters often display both types of interactions with activator proteins?

Answer 4

yes

Question 5

what can repression be viewed as an effect of

Answer 5

that it prevents RNA polymerase from binding to the promoter

Question 6

how is repression achieved

Answer 6

by steric hindrance

Question 7

what region do repressor proteins bind to

Answer 7

between -40 -> +20

Question 8

what does prevention of the RNA polymerase binding to the promoter in essence do?

Answer 8

prevents RPc formation

Question 9

what is the transcription bubble?

Answer 9

region within RNA polymerase where two DNA strands are separated

Question 10

what is there a region of within this bubble

Answer 10

RNA : DNA bubble