Transcription

Question 1

template strand

Answer 1

strand of DNA that is transcribed
-complementary to transcribed RNA

Question 2

non-template strand

Answer 2

exactly the same as transcribed RNA besides the T/U change

Question 3

consensus sequence

Answer 3

sequence within the promoter that is recognized by sigma factor or TF

Question 5

sigma factor and TF

Answer 5

once it recognizes consensus sequence, it promotes RNA polymerase at transcription start site

Question 6

terminator site

Answer 6

DNA sequence of terminator causing polymerase to pause

Question 7

Rho utilization (rut) site

Answer 7

sequence upstream of terminator site that is a binding site for rho protein that stops transcription
- rich in cytosine

Question 8

TATA binding protein (TBP)

Answer 8

general transcription factor that is essential for all RNA pol II transcription

Question 9

TFIIX

Answer 9

general transcription factor that recruits RNA pol II to promoter sequence, similar function to sigma factor

Question 10

mRNA is processed by

Answer 10

-5’ cap
-poly(A) tail addition
-splicing out introns