transcription Flashcards
Lac operon
CAP
Lac repressor
RNA pol
polycistronic transcription
multiple protein regulate by same promoter
Clamp domain
Clamp domain provides processivity to the RNA polymerase
The clamp domain of the Rpb1 subunit of RNA Pol II provides stability anchoring it to DNA
It is in an open conformation when RNA Pol II binds initially and shuts when elongation starts. Increases the processivity.
Yeast RNA polymerase ii
Rbp 1 2: similar with prokryotes
Rbp 4 7: additional
Initiator
bind TFII D
BRE
upstream TATA box
TFII B recognition element
DPE
bind with other subunits of TFIID
present in promoters with initiator element
CpG islands
100-1000
5’C-G
no consensus sequence
multiple start site and bidirection
Enhancer
far away from promoter
200bp (several 6-12 bp functional sequence)
up/down stream
may help to generate, stabilize, and increase the rate of transcription within the chromatin loops
UAS
yeast enhancer
SMC
Structural Maintenance of Chromosomes proteins
generat loops
linker scanning mutations
common reporters
-Green Fluorescent protein (GFP)
-β-galactosidase (lacZ)
-thymidine kinase (tk)
-luciferase (luc)
-chloramphenicol acetyltransferase (CAT)
recruitment of RNA pol
TBP (bind TATA box and DNA minor groove)
TFIID (bind Inr and DPE)
TFII A (associate upstream)
TFII B (associate C terminal of TBP and major groove)
TFII F and Pol II (form Core PIC)
TFII E (binds and stabilizes the subunits of the core PIC)
TFII H (bind through interaction with TFII E, form the pre-initiation complex–close PIC)
TFII H (subunit XPB use ATP melting DNA, result in open PIC)
Core PIC
TBP
TFII D
TFII A
TFII B
TFII F and Pol II
