DNA replication

Question 1

helicase (eu)

Answer 1

CMG helicase:
hexomer of MCM with Cdc 45 and GINS complex

bind with leading strand

Question 2

RPA

Answer 2

replication protein A
bind ssDNA, prevent secondary structure

Question 3

leading strand extension

Answer 3

primase/DNA pol alpha complex: form primer (RNA and DNA)
pol alpha extend the primer

DNA Pol epsilon extension

Question 4

lagging strand extension

Answer 4

primase/DNA pol alpha complex: form primer (RNA and DNA)
pol alpha extend the primer

Pol delta/PCNA complex replace, extend the Okazaki fragment

Ribonuclease H and FEN-1:
Ribonuclease H recognize the hybrid primer
FEN-1 displace primer, and helps remove the primer completely
Pol delta: replace primer with DNA

DNA ligase ligate fragment

Question 5

what molecule make pol stable on template

Answer 5

PCNA
Rfc: open PCNA and load RCNA it to primer

Question 6

replication origins

Answer 6

AT-rich
marked by origin recognition complex （ORC）

Question 7

ORC

Answer 7

six subunit protein
load helicase aided by other initiation factor

Question 8

when does MCM load

Answer 8

G1 phase (inactive form)
active in S phase (by phosphorylation and interaction with other proteins)

Question 9

DNA replication from replication origins is ()directional

Answer 9

bidirectional