Transcription Flashcards

1
Q

The Central Dogma

A

information flows in one direction: from DNA to RNA to proteins

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2
Q

Central Dogma includes three processes

A

Replication – copy of DNA is made
Transcription – DNA to RNA
Translation – RNA to proteins

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3
Q

DNA contains genes
genes are

A

small sections of DNA with the blueprint for making proteins

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4
Q

DNA is located in

A

the nucleus

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5
Q

Proteins are ____ which are located in the cytoplasm

A

made on the ribosomes

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6
Q

There must be another molecules that helps bring the genetic code ____________________________________

A

outside the nucleus to the ribosomes

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7
Q

The special helper molecules is _______ = _____________

A

RNA = ribonucleic acid

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8
Q

Transcription is ______ _________________________________

A

the process by which RNA is built from a template of DNA

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9
Q

Transcription converts

A

a gene into a single- stranded RNA molcules

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10
Q

Steps of Transcription

A

Inititation An enzyme (RNA polymerase) unzips the DNA

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11
Q

The enzyme recognizes the start of a gene called a ________ and binds to it

A

a promoter

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12
Q

Elongation

A

The addition of nucleotides to the mRNA strand

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13
Q

RNA polymerase does what?

A

reads the unwound DNA strand and builds the mRNA molecule, using complementary base pairs.

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14
Q

The promoter signals the DNa to wind so the ____ can what?

A

(enzyme)
read the nitrogen bases

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15
Q

During this process of RNA polymerase, ____________in the DNA binds to a _______ in the ____

A

an adenine (A)
uracil (U)
RNA

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16
Q

Termination

A

The ending of transcription

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17
Q

Occurs when RNA polymerase crosses a ______________ sequence in the gene.

A

stop (termination)

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18
Q

The ______ recognizes the start of a gene, called the promoter, and binds to it

A

enzyme

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19
Q

The mRNA strand is complete, and it ______ _____

A

detaches from DNA

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20
Q

messenger RNA (mRNA)

A

carries the message that will be translated to form a protein

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21
Q

Ribosomal RNA (rRNA)

A

forms part of ribosomes where proteins are made

22
Q

Transfer RNA (tRNA)

A

brings amino acids from the cytoplasm to a ribosome

23
Q

Translation

A

process that converts an mRNA message into a protein (polypeptide)

24
Q

Proteins are what?

A

made of amino acids linked together by peptide bonds

25
And proteins are ....
made by ribosomes
26
Ribosomes
consist of two subunits
27
The large subunit ......
has three binding sites for tRNA
28
The small subunit .......
binds to mRNA
29
Strands of mRNA
read in groups of three nitrogen bases
30
3 nitrogen bases=
codon
31
A codon is
a sequence of three nitrogen bases that codes for an amino acid
32
There are
64 different codons
33
There are how many different amino acids
20
34
Genetic Code
is a chart that matches each codon to its amino acid
35
There are _____ ______ chart versions of the genetic code
MANY different
36
Many amino acids are coded for by ______ _________
more than one codon
37
Leucine is coded for by
UUA, UUG, CUU, CUC, CUA, and CUG
38
Some codons do not code for amino acids:
three STOP codons (UAA, UAG, UGA)
39
Some codons do not code just for amino acids
one START codon (AUG = methionine)
40
Regardless of the organism codons always…
code for the same amino acid
41
The change in ....
the order of the codons and the order they are read changes the protein
42
anticodon
is a set of three nucleotides that is complementary (opposite) to an mRNA codon
43
Anticodons are carried by
tRNA
44
This ensures that
the correct amino acid will be added to the growing polypeptide chain
45
INITIATION
mRNA travels and binds to the small ribosomal subunit
46
tRNA brings the amino acid methionine _____ ___________
to bind to the start codon
47
Amino acids are brought to the ribosome by tRNAs and linked together with _____ ______ to form a chain
peptide bonds
48
When a ______ _____ is recognized, the elongation of amino acid chain terminates The ribosome then folds the polypeptide into its final structure The ribosome will then disassemble
STOP codon
49
mutation
any change in an organism’s DNA sequence
50
Some mutations are caused by environmental factors called?
mutagens
51
Any factor that causes a change in DNA is called
mutagen
52