transcription

Question 1

explain the functions of RNA polymerase

Answer 1

• 1: makes ribosomal RNA
• 2: makes most RNA transcripts e.g. mRNA and miRNA
• 3: makes tRNA and other small RNAs

Question 2

define 3 stages of transcription

Answer 2

• initiation
• elongation
• termination

Question 3

what is the promoter

Answer 3

• region of DNA where transcription is initiated
• contain a TATA box
• control the binding of RNA polymerase to DNA

Question 4

steps in initiation

Answer 4

-RNA polymerase is recruited to the promoter TSS by transcription factors
- transcription bubble is formed by unwinding the template

Question 5

steps in elongation

Answer 5

• RNA polymerase uses the DNA as a template
• RNA transcripts are made from 5’ to 3’
• new nucleotides are added to the 3’ end

Question 6

steps in termination

Answer 6

• RNA drops off the DNA template by capping at the 3’ end