Transcription

Question 1

What is the coding strand?

Answer 1

It is the DNA strand that is identical to the mRNA strand being synthesized

Question 2

what is the template strand?

Answer 2

It is the DNA strand that is complementary and anti-parallel to the mRNA being synthesized

Question 3

holoenzyme

Answer 3

PROKARYOTES; a sigma factor with core enzymes; it recognizes and binds to the promotor during initiation then the sigma factor falls off and the core enzymes continue

Question 4

RNA pol I

Answer 4

EUK; transcribes precursors to rRNA

Question 5

RNA pol II

Answer 5

EUK; transcribes precursors to mRNA

Question 6

RNA pol III

Answer 6

EUK; transcribes precursors to tRNA

Question 7

what are the 2 sequences recognized by sigma factor in prokaryotes?

Answer 7

TATA box (pribnow box) and -35 sequences

Question 8

what are the 2 sequences RNA pol II recognizes in Eukaryotes?

Answer 8

TATA box (Hogness box) and CAAT box

Question 9

systemic lupus erythematosus

Answer 9

pt produces Ab to snRNP’s and nucleic acids (butterfly rash)

Question 10

Amanita phalloides mushroom

Answer 10

Alpha amanitin toxin binds to RNA pol II

Question 11

What are the two types of termination in Prokaryotes?

Answer 11

Rho independent (GC stem loop and poly U stretch) and Rho dependent (rho factor)