Totipotency& Tissue Culture

Question 1

Equipment (11 things)

Answer 1

White mustard seeds, agar, distilled water, damp sponge, cling film, McCartney bottles, weighing scales, plastic tray, 250ml beaker, glass rod, scissors

Question 2

Method: (5 steps)

Answer 2

1. Sprinkle seeds on damp sponge&to germinate& use when just starting to unfold their cotyledons
2. make agar jelly, pour in McCartney bottles and allow to set
3. With sharpe scissors cut the tops off just below the shoot apex (explants)
4. push the stem of the explant into the gel
5. Place on sunny window sill&observe over 10 days

Question 3

Evaluation issues: (2 things)

Answer 3

Unwanted pathogens growing in agar gel

Wrong part of the plant cute& inserted into gel

Question 4

Outcome expected:

Answer 4

Plant roots and leaves to continue growing