TOPIC B2 Cell Organisation Flashcards
What is a tissue?
A group of similair cells that work together to carry out a function.
What is an Organ?
An organ is a group of different tissues that work together to perform a certain function.
Give an example of an organ:What tissues is it made up of?
STOMACH. Made up of muscular, epithelial and glandular tissues.
What is an organ system?
An organ system is a group of organs working together to perform a function.
Give an example of an organ system:
THE DIGESTIVE SYSTEM. It breaks down and absorbs food.
Organ systems work together to make…
Enitre organisms.
What does the salivary glands do?
Produce amalyse enzyme in the saliva.
What does the liver do?
Produces bile. Bile neutrslises stomach acid and emulsifies fats.
What does the large intestine do?
Absorbs water from undigested food, leaving faeces (poo).
What does the stomach do? (3)
- It pummels the food with its muscular walls.- Produces the protease enzyme “pepsin”- Produces hydrochloric acid to kill bacteria and to give the right PH for protease enzyme to work - PH 2.
What does the pancreas do?
Produces protease, amalyse and lipase enzymes and releases them into the small intestine.
What does the small intestine do? (2)
Produces protease, amalyse and lipase enzymes to complete digestion.Also where the digested food is absorbed out of the digestive system into the blood.
Living things have tons of reactions going on inside their cells. What controls these reactions?
ENZYMES - LARGE PROTEINS.
What do enzymes do?
They speed up reactions inside living things by acting as catalysts.
What is a CATALYST?
A catalyst is a substance which increases the speed of a reaction without being changed or used up in the reaction.
EXPLAIN THE LOCK AND KEY MODEL:
Every enzyme has an active site and a unique shape. The substance involved in the reaction HAS TO FIT into the active site for the enzyme to work.
How does temprature effect the rate of a reaction involving an enzyme?
A higher temprature speeds up the rate at first but if it gets too hot, some of the bonds holding the enzyme together break. This changes the shape of the enzyme’s active site so the substrate won’t fit anymore - the enzyme has denatured.
What is an OPTIMUM TEMPRATURE?
The temprature that the enzyme works best at.
How can PH effect the rate of a reaction involving an enzyme?
If the PH is too high or low, it affects the bonds holding the enzyme together. It changes the shape of the active site and denatures the enzyme.
What is an OPTIMUM PH?
The PH that the enzymes work best at?
INVESTIGATING THE EFFECT OF PH ON ENZYME ACTIVITY:Give the 7 steps:
1) Put a drop of iodine solution into every well of a spotting tile.
2) Place a bunsen burner on a heatproof mat and a tripod and gauze over it. Put a beaker on top and heat the water until it is 35c.
3) Use a syringle to add 1cm cubed of amalyse solution and 1cm cubed of a buffer solution with a PH of 5 to a boiling tube. Put the tube into the beaker of water and wait for 5 mins (with test tube holders)
4) Use a different syringle to add 5cm cubed of a starch solution to the boiling tube and immediently mix the contents of the boiling tube and start a stop clock.
5) Use continous sampling to record how long it takes for the amalyse to break down all of the starch., use a dropping pipette to take a fresh sample from the boiling tube every 30 seconds and put a drop into a well.
6) When the iodine solution remains browny orange, there is no longer starch.
7) Repeat it with buffer solutions of different pH values to see how pH effects the time taken for the starch to be broken down.
Rate is a measure of…
How much something changes over time.
FORMULAE TO FIND RATE OF REACTION:
RATE OF REACTION = 1000 DIVIDED BY TIME.
Digestive enzymes break big molecules down into smaller ones. Give examples of BIG MOLECULES:
- FATS.- STARCH.-PROTEIN.
Give an example of a CARBOHYDRASE (Something that breaks down starch):
Amalyse.
Where is amalyse made? (3)
Salivary Glands.Pancreas.Small Intestine.
Where does amalyse work? (2)
MOUTH.SMALL INTESTINE.
What does PROTEASE BREAK DOWN?
Proteins.
What does amalyse break down starch into?
Sugar.
What does PROTEASE break down protein into?
Amino Acids.
Where are Protease made? (3)
- Stomach.- Pancreas.- Small Intestine.
Where do Protease work (2)
StomachSmall Intestine
What do Lipase break down?
LIPIDS - FATS.
Where are Lipase made? (2)
PancreasSmall Intestine
Where do Lipase work?
Small Intestine
What do Lipids turn into?
Glycerol and fatty acids.
Where is Bile produced?
In the liver.
Where is Bile stored?
In gall bladder before it is released into the small intestine.
Is Bile Alkaline?
Yes, it neutralises hydrochloric acid (from the stomach) and makes conditions alkaline.
The enzymes in the small intestine work best in….
alkaline conditions.
Bile emulsifies Fats. What does this mean?
It breaks the fat down into tiny droplets. Gives a bigger surface area of fat for lipase to work on = makes digestion faster.
4 steps to prepare a food sample:
1) Get a piece of food and break it up using a pestle and mortar.
2) Transfer the ground up food to a beaker and add some distilled water.
3) Give the mixture a good stir with a glass rod to dissolve some of the food.
4) Filter the solution using a funnel lined with filter paper. This will get rid of the solid bits of food.
What test is used for sugar?
BENEDICT’S TEST.
What test is used for starch?
IODINE SOLUTION.
What test is used for proteins?
BIURET TEST.
What test is used for lipids?
SUDAN 111 TEST.
How do you know if there is sugar in a solution?
The solution will change from the normal blue colour to green, yellow or brick red - depending on how much sugar is in the food.
How do you know if there is starch in a solution?
The colour of the solution will change from browny orange to black or blue-black.
How do you know if there is protein in a solution?
The solution will change from blue to pink or purple.
How do you know if there is lipids in a solution?
The mixture will seperate out into two layers, the top layer will be bright red.
Give the 4 stages of the BENEDICT’S TEST FOR SUGAR:
1) Prepare a food sample and transfer 5cm cubed to a test tube.2) Prepare a water bath so that it’s set to 75.c.
3) Add 10 drops of Benedict’s solution to the test tube using a pippette.
4) Place the test tube into the water bath with a test tube holder, leave it there for 5 mins. Look at the colour.
Give the 2 stages of the IODINE SOLUTION FOR STARCH:
1) Make a food sample and transfer 5cm cubed to a test tube.2) Add a few drops of iodine solution and gently shake the tube to mix the contents. CHECK COLOUR.
Give the 2 stages of the BIURET TEST FOR PROTEINS:
1) Prepare a sample of your food and transfer 2cm cubed to a test tube.
2) Add 2cm cubed of biuret solution to the sample. Mix the contents of the tube by gently shaking. CHECK COLOUR.
Give the 2 stages of the SUDAN 111 TEST FOR LIPIDS:
1) Prepare a food sample but don’t filter it and transfer 5cm cubed to a test tube.
2) Add 3 drops of SUDAN 111 Stain solution to the test tube and gently shake.
3) Check colour.
The air that you breathe in goes through the…
Tranchea then it passes through the bronchi then the bronchioles and ends up in the alveoli.
Alveoli in the lungs are surrounded by blood capillaries. Explain.
Blood comes into the lungs through the capillaries. Contains a lot of carbdon dioxide and very little oxygen.
How does diffusion work in the alveoli?
The oxygen diffuses out of the air in the alveolus (where there is a high concentration) into the blood (where there is a lower concentration).
What is the breathing rate?
How fast a person moves air in and out of their lungs.
How to calculate breaths per minute?
number of breaths divided by number of minutes.
What 3 things is the circulatory system made up of?
Blood VesselsBloodHeart
What is a double circulatory system?
Two circuits joined together.
What happens in the first circuit in the heart?
The heart pumps deoxygenated blood to the lungs . The blood picks up oxygen in the lungs. Oxygenated blood returns to the heart.
What happens in the second circuit in the heart?
The heart pumps oxygenated blood around all the other organs of the body. This delivers oxygen to the body cells. Deoxygenated blood returns to the heart to be pumped out to the lungs again.