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PMLS P3 > TOPIC 8 > Flashcards

TOPIC 8 Flashcards

1
Q

In 1974, the CDC published

A

Classification of Etiological Agents on the
Basis of Hazard.

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2
Q

National Institutes of Health (NIH)
published

A

the NIH Guidelines for
Reasearch Involving Recombinant DNA
Molecules.

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3
Q

CDC and NIH jointly published

A

the
Biosafety in Microbiological and
Biomedical Laboratories (1984)

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4
Q

is the containment principles,
technologies, and practices that are
implemented to prevent unintentional
exposure to pathogens and toxins, or
their accidental release.

A

Biosafety

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5
Q

(1966) created
the biohazard
symbol.

A

Charles Baldwin

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6
Q

14 CLASSIFICATION
OF BIOLOGICAL
MATERIALS

A

-Blood
-Body fluids
-Cerebospinal fluid
-abcess
-urine
-bone
-eye
-ear
-Foregin track
- GI Track
- Reproductive system
-Genital system
-stool
-Tissue

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7
Q

A collection manual must have

A

-Safety considerations
-Selection
of
the
appropriate
anatomic site and specimen
-Collection instruction
-Transportation instruction
-Labeling instruction
-Special instruction
-Sterile vs. Nonsterile collection
-Minimal acceptable quality and
recommended quality

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8
Q

aerobes

A

Needle Aspiration, Swab

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9
Q

Anerobes

A

Needle Aspiration, Tissue

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10
Q

Urine, CSF, Serous fluid, stool and sputum

A

Sterile container

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11
Q

Throat, nose, eyes wound and abscess

A

Sterile cotton tipped applicator

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12
Q

Dacron and Rayon

A

Good for bacteria and viruses

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13
Q

Dacron

A

Good for viruses

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14
Q

Cotton swab with charcoal

A

Use for urogenital swab

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15
Q

4°c

A

Refrigerator temperature

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16
Q

22°c

A

Ambient or room temperature

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17
Q

37°c

A

Body temperature

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18
Q

20-70°C

A

Freezer temperature

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19
Q

Specimens should be completely
labeled with:

A

-Patient’s name
-Identifying number
-Birthdate
-Date and time of collection
-Source
-Initials of the individual that
collected the sample

20
Q

A device that encloses a working area to
protect workers from aerosol exposure and
infectious disease agents

A

BIOLOGICAL SAFETY CABINETS

21
Q

In BSC, the air contains the infectious
material is sterilized, either by

A

heat, UV
light, or by passage through a high
efficacy particulate air (HEPA) resistance filter

22
Q

It is an open-fronted type of cabinet with
negative pressure (ventilated cabinets)

A

CLASS 1 BIOSAFETY CABINET

23
Q

It allows room (unsterilized) air to enter the
cabinet, cabinet, circulate around the area
and exposure the material within; only the air
to be exhausted is sterilized using HEPA
filter

A

CLASS 1 BIOSAFETY CABINET

24
Q

-also known as the laminar flow BSC
commonly used BSC in a clinical
microbiology laboratory (Class IIA).
- It sterilizes the air using HEPA filter that
flows over the infectious material and the air
to be exhausted

A

CLASS 2 BIOSAFETY CABINET

25
Q

two types of Class II Cabinets

A

CLASS 2 A
CLASS 2 B

26
Q

has fixed openings; 70% of
the air is recirculated

A

CLASS 2 A

27
Q

variable sash opening;
used for chemicals, radioisotopes,
and
carcinogen

A

CLASS 2 B

28
Q

Most hospital clinical microbiology laboratory
technologists use

A

CLASS 2 BSC

29
Q

It provides the highest level of safety to the
worker.
The air coming into and going out of the
cabinets is sterilized using HEPA filter and
the infectious material within is handled with
rubber gloves that are attached and sealed
to the cabinet

A

CLASS 3 BIOSAFETY CABINET

30
Q

Used for BSL 4 agents

A

CLASS 3 BIOSAFETY CABINET

31
Q

These are agents that have no known potential for infecting
healthy people.

A

BIOSAFETY LEVEL 1 AGENTS

32
Q

This containment level is used in laboratory activities of
students (for academic purposes).

A

BIOSAFETY LEVEL 1 AGENTS

33
Q

Some examples of pathogens that requires this containment
level are Bacillus subtilis and Naegleria gruberi

A

BIOSAFETY LEVEL 1 AGENTS

34
Q

agents acquired by ingestion or exposure to percutaneous or
mucous membrane
include all the common agents of infectious diseases
In handling these agents, access to the laboratory is limited.

A

BIOSAFETY LEVEL 2 AGENTS

35
Q

The personnel handling these agents should also receive
immunization.

A

BIOSAFETY LEVEL 2 AGENTS

36
Q

Some examples of pathogens that require this containment level are HIV,
Bacillus anthracis, Yersinia pestis, Salmonella, and Shigella.

A

BIOSAFETY LEVEL 2 AGENTS

37
Q

These are potential agents for aerosol transmission
In processing these lethal pathogens, the air movement in the
laboratory must be controlled to contain the infectious materials

A

BIOSAFETY LEVEL 3 AGENTS

38
Q

Some examples of pathogens that require this containment level
are Mycobacterium tuberculosis, Francisella tularensis, Brucella
spp., Coxiella burnetti, St. Louis encephalitis virus, and systemic
fung

A

BIOSAFETY LEVEL 3 AGENTS

39
Q

These are agents that cause life-threatening infections
In handling these organisms, maximum containment and
decontamination of all personnel and materials before
leaving the area are observed.
Aerosol transmission with pressure is possible

A

BIOSAFETY LEVEL 4 AGENTS

40
Q

Some examples of pathogens that require this
containment level are arbovirus, arenavirus, filovirus,
and smallpox virus.

A

BIOSAFETY LEVEL 4 AGENTS

41
Q

a policy of treating all
blood, tissue, body fluids
and OPIM (other potentially
infectious materials as
infectious)

A

UNIVERSAL PRECAUTION

42
Q

involve taking physical steps to isolate or
remove any possible pathogen hazards
from the workplace

A

Engineering and work practice control

43
Q

-gloves must be worn when coming in contact
with specimens and other hazardous
chemicals and OPIM
-gloves must be replaced every after one
patient and when the protective integrity is
compromised
-Eye and face protection items
-Protective body clothing (laboratory gowns,
aprons, laboratory coats, surgical caps, shoe
covers and disposable arm sleeves)

A

Personal protective equipment

44
Q

Must ensure that the workplace
is maintained in a clean and
sanitary condition

A

Housekeeping Techniques

45
Q

generally, refers to
contamination of our food or
environment with
microorganisms. - this means
bacteria, viruses, fungi, and
parasites.

A

Biological contamination

46
Q

describes the event at which a
foreign material or substance
that
illnesses
material.
can cause foodborne
food
e

A

Food contamination

47
Q
A

PMLS P3 (4 decks)

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