Topic 5 Flashcards
Thylakoids Membrane
-folded membrane
- contains photosynthetic proteins (chlorophyll)
- electron carrier proteins embedded in membranes
- both proteins involved in LDR
Stroma
- fluid centre
- contains enzymes involved in LDR
Inner and outer membrane of chloroplast
Control what can enter and leave the organelle
Where do LDRs and LIRs take place and what are they part of
- LDR = thylakoids membrane or grana
- LIR = stroma
- photosynthesis two stage reaction - LDR and LIR
LDR
- first stage
- requires light
- light energy and water used to create ATP and reduced NADP needed for LIR
Stages of LDR
- Photolysis
- Photoionisation of chlorophyll
- Chemiosmosis
- Production of ATP and reduced NADP
Photolysis of Water
-photolysis = light splitting
- light energy absorbed by chlorophyll and splits water into oxygen, H+ and e- (H2O - 1/2O2+2e-+2H+)
- H+ picked up by NADP to form NADPH - used in LIR
- passed along chain of electron carrier proteins
- oxygen used for either respiration or diffuses out of leave via the stomata
Photoionisation of chlorophyll
- light energy absorbed by chlorophyll
- electrons become excited and leaves the chlorophyll
- so chlorophyll has been ionised by light
- some energy released used to form ATP and reduced NADP in chemiosmosis
Chemiosmosis
- Electrons that left chlorophyll go along series of proteins embedded in thylakoid membrane
- As they move along they release energy and pump protons across the chloroplast membranes
- Electrochemical gradient created and protons pass through ATP synthase - produces ATP
- Protons combine with NADP (coenzyme) to become reduced NADP. Protons move from high to low concentration gradient - Chemiosmosis
LIR
- Calvin Cycle
- Stroma - contains enzyme called RuBisCo which catalyses the reaction
- temp sensitive as it involves enzymes
- uses CO2, reduced NADP and ATP to form a hexose sugar
Calvin Cycle
- ATP hydrolysed - energy
- reduced NADP donates hydrogen to reduce molecules GP
- CO2 reacts with ribulose phosphate - forms two molecules of glycerate 3-phosphate (3 carbon compound)
- GP reduced to triode phosphate using energy from ATP and accepting reduced NADP
- some carbon leaves from TP to turn into useful organic substances
- Rest of molecule used to regenerate RuBP
Limiting Factors
- reduces rate of photosynthesis
- temp, CO2 concentration and light intensity
Aerobic Respiration
- Glycolysis (cytoplasm)
- Link Reaction (mitochondrial matrix)
- Krebs Cycle (mitochondrial matrix)
- Oxidative Phosphorylation (mitochondrial inner membrane - cristae)
- produces ATP
Glycolysis
-1st stage aerobic and anaerobic
1. Phosphorylation glucose to glucose phosphate, using ATP
2. Production of TP
3. Oxidation of TP to produce pyruvate with a net gain of ATP and reduced NAD
- Glycolysis (cytoplasm)
- Link Reaction (mitochondrial matrix)
- Krebs Cycle (mitochondrial matrix)
- Oxidative Phosphorylation (mitochondrial inner membrane - cristae)
- produces ATP
Link Reaction
- pyruvate and NADH actively transported to mitochondrial matrix
- pyruvate oxidised to acetate
- NAD picks up hydrogen and becomes reduced NAD
- acetate combines with coenzyme A - Acetylcoenzyme A
Krebs Cycle
- AcetylCoA reacts with 4C molecule releasing CoA and producing a 6C molecule
- series of redox reactions - generates reduced coenzymes and ATP by substrate level phosphorylation and CO2 is lost
- 3x reduced NAD, 1x reduced FAD, 1x ATP, 2xCO2
Oxidative Phosphorylation
- most ATP made during this stage
- electron transfer chain
- movement of protons across cristae
- catalysed by ATP synthase
Anaerobic Respiration
- absence of oxygen, occurs in cytoplasm only
- pyruvate produced in glycolysis reduced to form ethanol and CO2 (in plants and microbes) or lactate (in animals) by gaining hydrogen from reduced NAD
- NAD can be reused in glycolysis and ensures more ATP is produced
Energy Transfer
- in any ecosystem, plants are producers - able to produce own food (carbs) using CO2 and water
- majority of energy lost between each tropic level via respiration and excretion. Remaining used to form biomass.
- amount of biomass remaining in organism measured in mass of carbon or dry mass of tissue per given area
Net Primary Production and Gross Primary Production
- GPP - chemical energy store in plant biomass in given area. Total energy resulting from photosynthesis.
- NPP - chemical energy store in plant biomass taking into account energy lost from respiration (R)
- NPP = GPP-R
Net Production of Consumers (animals)
- N=I-(F+R)
- I = chemical energy store in ingested food
- F = chemical energy lost to environment in faeces and urine
- R = respiratory losses
Nitrogen Cycle
- Saprobiotic nutrition and microbes
- Ammonification
- Nitrification
- Nitrogen Fixation
- Denitrification
Importance of Nitrogen Cycle
- Plants and animals cannot obtain nitrogen through gas exchange
- Nitrogen gas (N2) contains a triple bond
- Microorganims needed to convert N2 into nitrogen containing substances that plants and animals can absorb - proteins, ATP and nucleic acids all contain nitrogen
Phosphorus Cycle
- phosphorus used for DNA/RNA,ATP,Phospholipid bilayer - essential element for life
- different to carbon and nitrogen cycle as it is not found in gas (atmosphere)
- mainly found as phosphate ion, in mineral form in sedimentary rocks
Mycorrhizae
- fungal associations between plant roots and beneficial fungi
- beneficial for plant growth:
1. Increases SA for water and mineral absorption
2. Acts like sponge - holds water and minerals around the roots
3. Makes plants more drought resistant and able to take up more inorganic ions. - role in nutrient cycles - improving uptake if relatively scarce ions (phosphate ions) - mutualistic relationship
Fertilisers
- added to soil to replace nitrate and phosphate ions lost when plants are harvested and removed from nutrients cycles as crops
- either natural (manure) - cheaper, but minerals and proportions can’t be controlled
-artificial (inorganic chemicals) - exact proportions of minerals, more water soluble so more ions dissolve in water surrounding the soil
Leaching
- water soluble compounds washed away, often into rivers/ponds
- causes eutrophication
Eutrophication
- nitrate leached from fertilised fields stimulates growth of algae in ponds (algal bloom)