Topic 4 - The Electron Microscope Flashcards
1
Q
What is the light source of the electron microscope
A
Electron gun (Tungsten filament)
2
Q
Where are electrons made into a parallel beam?
A
The Wenhelt cylinder
3
Q
How thin are specimens in an electron microscope?
A
60-90nm
4
Q
Difference between a scanning and transmission electron microscope.
A
Scanning is used for thicker specimens.
5
Q
Which method of fixation is used for EM?
A
Perfusion - Injecting fixative into a live animal’s heart.
6
Q
What are the sections made with for EM?
A
A Vibratome
