Topic 3 - Preparation of tissues for light microscopy Flashcards
1
Q
Which chemicals are normally used for fixation?
A
Aldehyde, Alcohol, Heavy metal salts, Acids, Formaldehyde.
2
Q
How are fixed samples rinsed?
A
Flowing tap water/Solvent
3
Q
What is ‘Embedding’?
A
Setting the sample in paraffin wax.
4
Q
How are sections made?
A
With a microtome
5
Q
What does Alcian Blue stain?
A
Polysaccharides
6
Q
What is stained by Azan?
A
Collagen Fibres
7
Q
What is stained by PAS?
A
Polysaccharides
8
Q
What is stained by Resorcin-fuchshin?
A
Elastic Fibres
9
Q
What is stained by Silver Impregnation?
A
Reticular Fibres
10
Q
What is stained by Schmorl?
A
Bone
