Topic 4-Exchange and transport Flashcards
Body mass and oxygen consumption
-Smaller animals consume more oxygen
-larger SA:V ratio
-lose more heat by respiration
-but need to maintain body temp
Effect of temperature on membrane permeability
-permeability increase as temperature increase due to the kinetic energy increase of the molecule
-Phospholipids gain kinetic E leading to more movement so membrane more FLUID
-Proteins denature due to extreme temp so permeability decreases
Requirements for active transport
Cell membrane, ATP, carrier protein
Importance of phospholipids changing according to temperature
-membranes control permeability
-such as O2 uptake
-so they need to be fluid
Effect of ethanol in cell membrane
-Disrupts phospholipid membrane
-Makes it more permeable
Control in cell membrane practicals
-Same type/age/part of plant
-same core borer used
-control temp/ph
-same V of solution, conc, etc
Diffusion of charged/uncharged particles
Uncharged-can diffuse through hydrophobic fatty acid tail
-Charged-facilitated diffusion
Effect of sucrose and glucose
-osmotic potential of 3% sucrose is equal to 6% glucose
-As sucrose has double molecular mass
-it is the no. of molecules that determine osmotic potential
Ψ=P+π equation
Water enters cell by osmosis:
-Ψ of cell <outside
-P increase until equal to π
-Ψ becomes equal to 0
Osmotic potential-plants
-Can’t directly measure as it includes turgor pressure and water potential
-can only be measured through incipient plasmolysis
-which is when P=0
Improvements in cell membrane practicals
-Full submerge plant in the solution so all surfaces are in contact
-control temperature
-use smaller intervals of concentration
-blot to remove excess solution
-repeat and calculate the mean excluding the anomalies
Determining osmotic potential from a graph of conc against plasmolysis
- Find conc with 50% plasmolysis
- Use a graph to figure out
Why are cells left for 10 mins
-to wait for water to move out
-so osmotic potential is accurate
Cell membrane with charged substances
-polar molecules are charged
-phospholipids have hydrophilic head and hydrophobic tail
-charged particles can’t pass through tail
-so need carrier proteins
How does membrane control movement
-Hydrophilic tail and hydrophobic head
-polar substances can’t pass through
-need carrier proteins