Topic 4 Flashcards
Describe how DNA is arranged in prokaryotes
In prokaryotic cells, DNA molecules are short, circular and not associated protein with proteins
Describe how DNA is arranged in eukaryotes
In the nucleus of eukaryotic cells, DNA molecules are very long linear and associated with proteins
Name the organelles, other than the nucleus, that contain DNA and describe how it is arranged here
Me mitochondria and chloroplasts of eukaryotic cells also contain DNA and like prokaryotic DNA it is shout circular and not protein associated
What is a chromosome?
DNA molecule associated with proteins
What is a gene and what doesit code for ( 2x bullet points)?
A gene is a base sequence of DNA that codes for:
- the amino acid sequence of a polypeptide
- a functional RNA (including ribosomal and tRNA)
What is meant by saying the genetic code is universal?
The same base triplets code for the same amino acids in all organisms (with some exceptions)
What is a locus?
The position a gene occupies on a particular DNA molecule.
What is a maple and what does it code for?
A sequence of three DNA bases that code for a specific amino acid
What is meant by saying the genetic code is non-overlapping?
Each base is only read once as apart of a specific triplet
What is meant by saying that the genetic code is degenerate?
Different base triplets /codons coding for the same amino acid
What is an exon?
Coding Sequences of DNA bases in a gene-they code for amino acid sequences
What is an intron?
Non-coding sequences of DNA bases in a gene
What is he genome?
The complete set of genes in a cell
What is a proteome?
The full range of proteins that a cell is able to produce
Describe the similarities between mRNA and tRNA
- Both are polymers of nucleotides/ polynucleotide strands
-nucleotide bases are adenine, uracil, guanine and cytosine - both contain ribose
- mom contain phosphodiester bonds
Describe the differences between mRNA and tRNA
- mRNA has a linear shape tRNA has a clover leaf shape
-mRNA does snot have hydrogen bands tRNA has hydrogen bonds - mRNA is variable in length, tRNA is not
-mRNA does not have an amino acid binding site, tRNA does
What happens in transcription?
1) Hydrogen bonds break
2) Only one DNA strand acts as a template
3) Free RNA nucleotides align based on complementary base pairing
4) In RNA uracil base pairs with adenine on DNA.
5) RNA polymerase joins adjacent nucleotides by phosphodiester bonds
7) Pre-mRNA is spliced removing introns to form mRNA
Where does transcription take place? (Eukaryotes)
In eukaryotes transcription occurs sin the nucleus.
How does transcription dissed eukaryotes and prokaryotes?
In prokaryotes transcription results directly in the production of mRNA from DNA
In eukaryotes transcription results in the production of pre-mRNA which is then spliced to form mRNA