Topic 3 Flashcards

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1
Q

What are the two type of cells?

A

Prokaryotic, eukaryotic

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2
Q

What is often prokaryotic?

A

Bacteria

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3
Q

What do prokaryotic cells not have?

A

Nuclei or membrane bound organelles

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4
Q

Features of prokaryotic cells

A

Ribosome, circular DNA, cell membrane, cell wall, cytoplasm, unfolds in membrane, plasmid, capsule, polo, flagellum.

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5
Q

Are prokaryotic or eukaryotic cells larger?

A

Eukaryotic

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6
Q

Structure and role of mitochondria?

A

Cristae folds, matrix inside. Used for aerobic respiration.

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7
Q

Structure and role of nucleus?

A

Nuclear envelope, nuclear pores, nucleolus, chromosomes. Synthesis of proteins.

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8
Q

Structure and role of nucleolus?

A

Dense within nucleus. Ribosomes are made.

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9
Q

Structure and role of rough endoplasmic rectillium.

A

Flattened membrane bound sacks with ribosomes attached. Proteins made an transported through endoplasmic rectillium.

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10
Q

Structure and role of ribosomes?

A

RNA and a protein. Protein synthesis.

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11
Q

Structure and role of smooth endoplasmic rectillium.

A

Flattened membrane bound sacks. Makes lipids and steroids.

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12
Q

Structure and role of Golgi apparatus?

A

Flattened membrane bound sacks, decreasing in size, vesicles. Modifies proteins and moves them into vesicles for transport.

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13
Q

Lysosomes structure and role.

A

Sacks of digestive enzymes, single membrane. Breaks down unwanted substances/structures.

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14
Q

Centriole roles and structure?

A

Hollow cylinder pairs, 9 microfibres each, forms spindle fibres.

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15
Q

What structures contain their own DNA?

A

Mitochondria, chloroplasts.

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16
Q

What is the process of making a protein?

A

DNA to tRNA to mRNA to ribosome, protein made,move through ER to get shape, vesicles formed, then fuses with Golgi apparatus, protein is modified, vesicles made, vesicles fuses with cell membrane, protein released. (Extra cellular eg enzyme)

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17
Q

How does the ovum move

A

Wafted along oviducts by ciliated cells and muscular contraction

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18
Q

What does the cytoplasm of the ovum contain?

A

Protein and lipid food stores for the embryo.

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19
Q

How do sperm get the energy to swim

A

A mitochondria inside

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20
Q

How to sperm move through the cell

A

Flagellum. muscular contraction. Chemicals released by ovum.

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21
Q

What is the acrosome reaction?

A

Acrosome swells and fuses with the surface membrane of sperm. It then releases digestive enzymes which breaks the zone pellucidia.

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22
Q

The acrosome is an example of what?

A

Lysosomes.

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23
Q

What is the cortical reaction?

A

The sperm fuses with and penetrates the cell membrane of the egg. The ovum releases chemicals which causes the zona pellucidia to thicken. This prevents other sperm from entering the egg.

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24
Q

What happens after the cortical reaction?

A

The sperm nucleus and the egg nucleus fuse to produce a fertilised egg.

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25
Q

What is a fertilised egg called?

A

A zygote.

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26
Q

What is the structure of the ovum?

A

Lipids, lysosomes, cytoplasm, haploid nucleus, cell membrane. Surrounded by the Zona pellucidia, his us all then surrounded by follicle cells from ovary.

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27
Q

Structure of a sperm?

A

Acrosome, haploid nucleus, mitochondria, flagellum.

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28
Q

What do the 23 pairs of chromosomes consist of?

A

22 homologous pairs and one sex pair

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29
Q

What is mitosis used for?

A

Cell repair and growth, asexual reproduction.

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30
Q

Where does meiosis happen?

A

Ovary, flowering plants, and testes

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31
Q

Why is meiosis good

A

It creates haploid cells and genetic variation.

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32
Q

What is independent assortment?

A

The way a pair of chromosomes line up

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33
Q

What is crossing over?

A

Chromosomes come together and 4 chromatids come into contact at contact points. The chromatic breaks, exchanges and rejoins the DNA of non sister chromatids.

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34
Q

What is the breaking point in crossing over called?

A

Chaisma

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35
Q

What is linkage of genes?

A

If a gene has a same locus on a chromatid they are likely to be passed on together.

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36
Q

Who gave evidence for linkage?

A

Gregor Mendel

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37
Q

What did Gregor Mendel show in his experiment. What could be concluded?

A

During f1 only one pair of characteristics were shown out of four. And f2 all four were shown. Genes were linked.

38
Q

What is sex linkage?

A

Genes that are passed on on y or X chromosomes.

39
Q

Example of sex linkage? Why?

A

Colour blindness in males. Recessive on X chromosome, but as males only have one x it becomes dominant

40
Q

What are the three stages of interphase?

A

G1, S, G2

41
Q

How does DNA condense?

A

DNA winds around and binds to histone proteins. These both cool to form chromatin fibre, this then starches to a protein scaffold on one side. This form loops of chromatin. This structure folds to make the condensed chromosome.

42
Q

What happens in interphase?

A

Protein synthesis due to unraveled chromosomes, DNA replication, organelles are synthesised.

43
Q

What happens in prophase?

A

Chromosomes condense, two chromatids per chromosome. (Four arms) joined at the centromere. Centrioles move to opposite ends of the cell and spindle fibres form between them. The widest part of the spindle is the equator. Nuclear envelope breaks down. Nucleolus disappears.

44
Q

What happens in metaphase

A

Centromeres attach to spindle fibres at the equator

45
Q

What happens in anaphase?

A

The centromeres split and spindle fibres shorten. This pulls a chromosome into two chromatids. These are pulled to opposite poles. Spindle fibres break down.

46
Q

What happens in telophase?

A

Chromosomes unravel, two nuclear envelopes form. Two sets of genetic material are now in separate nuclei.

47
Q

What happens in cytoplasmic division?

A

Ring of protein filaments contract and cell membrane constructs. Two cells are formed. In plants a new cell plate is formed.

48
Q

In a plant cell how is a cell wall formed?

A

Cytoplasmic division. The Golgi produces vesicles containing genetic material, the move along microtubioles and fuse

49
Q

How many replications does a zygote go through until it is totipotent?

A
  1. 8 total cells
50
Q

What does totipotent mean

A

It can develop into any cell

51
Q

How long do cells stay totipotent

A

Up to 14 days.

52
Q

What is a blastocyst

A

Hollow ball of cells

53
Q

When is a blastocyst formed

A

5 days after contraception

54
Q

What does the blastocyst form into

A

Outside turns into placenta and inner turns to embryonic tissue

55
Q

What type of cells are the inner cells of the blastocyst

A

Pluripotent

56
Q

What does pluripotent mean

A

They can form into most cells but not all

57
Q

What are multi potent cells

A

They can develop into similar cells of the same system

58
Q

Why can plants produce clones

A

They are totipotent and have the ability to differentiate.

59
Q

What is the process of tissue culture

A

Ex-plants are surface sterilised and placed on a agar medium containing nutrients and growth hormones.

These cells divide to form a mass of undifferentiated cells called a callus

The growth regulators can be altered to allow the callus to develop into a small group of cells

These then develop into plants that are genetically identical clones.

60
Q

Advantages and disadvantages of tissue culture

A

+ large number of plants very fast for food or desirable quality.

-less genetic variation, leading to easy extinction

61
Q

Where are stem cells for research taken from

A

Unused ivf

62
Q

What type of cells are stem cells used for research.

A

Pluripotent

63
Q

What could happen with stem cells and patents

A

Rejection

64
Q

What is therapeutic cloning

A

Nucleus from patients somatic cell put into an ovum. Electric shock. Diploid cell that acts as a zygote.

65
Q

What allowed more research into stem cells

A

Animal- human embryos

66
Q

What is ipsc

A

Somatic cells to pluripotent

67
Q

What is an animal human embryo

A

Human nucleus. Animal ovum.

68
Q

What does ipsc prevent.

A

Rejection and ethical issues.

69
Q

How was the nucleus shown to control cell development

A

Different Agal cells were grown. Hats were removed and stalks swapped. The intermediate heads that grew were a mix of the two species. The hate we’re then moved again and hates that coincided with the rhizoid grew

70
Q

What did the agal cell experiment show about the intermediate hats

A

mRNA carries the protein that control structure, that why the intermediate hats were mixed

71
Q

How was gene expression shown

A

mRNA was taken from undifferentiated cells and differentiated cells. The differentiated cells had complimentary DNA made, the mRna was then digested. The complimentary DNA and the mrna from the undifferentiated cell was mixed. Some double helixes formed which showed that only some genes were expressed at a time.

72
Q

What happens when genes are switched on

A

Mrna is produced which translates into a Specific protein.

73
Q

When the genes are switched off what is present? How does this work?

A

Methyl group

The group stops RNA polymerase bonding to the gene, this means no transcription so no mRNA

Histones are tightly wrapped in present of the methyl group. This tightness prevent the rna polymerase from bonding so no mRNA so no protein

74
Q

What is the epigenome

A

The types of epigenetic markers

75
Q

What is the genome

A

All the DNA containing a full set of genes

76
Q

What is the lac operon model with no lactose

A

When lactose is absent the repress of bonds to the operator so rna polymerase can not bind. The gene can then not be transcribed

77
Q

What is the lac operon model with lactose present

A

Lactose bonds to the depressor so rna polymerase can bind to promoter region. This allows transcription and translation so an enzyme so formed. Gene is on.
A regulator protein is also required

78
Q

What is polygenic inheritance. Distribution.

A

A number of genes are involved in the inheritance of a characteristic.
Continuous.

79
Q

What does multifactorial mean

A

Both genetic and environmental factors are involved.

80
Q

What is melanin

A

A dark hair pigment

81
Q

Where is melanin made

A

Melanocytes

82
Q

What activates melanin production

A

MSH

83
Q

What transports melanin

A

Melansomes

84
Q

What does more melanin give

A

Uv protection

85
Q

How do you have more melanin

A

Having more MSH receptors and

86
Q

What makes more MSH and receptors

A

Uv

87
Q

What makes hair lighten

A

Destruction of melanin

88
Q

What are oncogenes

A

Codes for proteins that simulates transition between cell cycle stages

89
Q

What happens if a gene has less methylation

A

It is continuously active

90
Q

What are tumour suppressor genes

A

Stop the cell cycle by producing proteins. Methylation turns gene off.

91
Q

What is p53

A

Stops the enzyme that allows g1 to s1 stage