topic 2 - cells Flashcards
name the organelles in eukaryotic cells
-nucleus
-cell membrane
-rough endoplasmic reticulum
-smooth endoplasmic reticulum
-golgi apparatus
-mitochondria
-ribosomes
-lysosomes
-cell wall
-permanent vacuole
-chloroplasts
Explain the structure and function of the nucleus
Contains genetic material and controls cell activity
-Nuclear envelope - double membrane that controls material entry/exit
-Nuclear pores
-Nucleoplasm
-Chromosomes - protein- bound. linear DNA
-Nucleolus - smaller sphere inside - site of rRNA production and makes ribosomes
Explain the structure and function of the endoplasmic reticulum
2 types of endoplasmic reticulum - system of folded membranes throughout the cytoplasm. Continuous with the outer nuclear membrane
-Rough endoplasmic reticulum - ribosomes on surface. Important in protein synthesis. Pathway to transfer proteins out of the cell
-Smooth endoplasmic reticulum - has no ribosomes. Synthesises and stores lipids and carbohydrates.
Explain the structure and function of the Golgi Apparatus
-Structure similar to smooth endoplasmic reticulum just more compact
-Folded membranes form cisternae
-Secretary vesicles pinch off from the cisternae
-Add carbs to proteins to form glycoproteins
-Produce secretory enzymes
-Secrete carbs
-Transport, modify and store lipids
-Form lysosomes
-Finished products transported to cell surface in vesicles where they fuse with the membrane and the content is released.
Explain the structure and function of lysosomes
-Collection of digestive enzymes
-Hydrolyse phagocytic cells
-Break down dead cells
-Digest worn out organelles for reuse of materials
-Release enzymes to outside of cell to destroy material
Explain the structure and function of mitochondrion
-Site of aerobic respiration and ATP production
-Contains DNA to code for enzymes needed in respiration
-Double membrane
-Inner membrane called the cristae which increases surface area for enzymes
-Fluid centre called the mitochondrial matrix
Explain the structure and function of ribosomes
Site of protein synthesis
-Small, made up of 2 sub-units containing protein and rRNA
-2 types of ribosome
-80S - large ribosome found in eukaryotic cells
-70S - smaller ribosome found in prokaryotic cells, mitochondria and chloroplasts
Explain the structure and function of the vacuole
Supports the cell by making it turgid
-filled with fluid surrounded by a single membrane called a tonoplast
-Temporary store of sugars and amino acids
Explain and structure and function of chloroplasts
Site of photosynthesis
-Surrounded by a double membrane
-Contains thylakoids (folded membranes embedded with pigment)
-Stack of thylakoids is called a granum
=Fluid-filled stroma - contains enzymes for photosynthesis
-Found in plants
Explain the structure and function of the plasma membrane
-Controls exit/entrance of molecules
-Found in all cells
-Phospholipid bilayer
What are the 3 types of microscopes?
optical, transmission electron, and scanning electron
What is magnification?
How many times larger the image is compared to the actual object
What is resolution?
The minimum distance between 2 objects in which they can still be viewed as separate. The resolution in an optical microscope is determined by the wavelength of light, and the resolution in electron microscopes is determined by the wavelength of the beam of electrons
Explain the features of an optical microscope
A beam of light is condensed to create the image
Poorer resolution due to light having a longer wavelength
Lower magnification
Colour images
Can view living samples
Explain the features of electron microscopes
A beam of electrons is condensed to create the image. Electromagnets are used to condense the beam
Higher resolving power as electrons have a short wavelength
Higher magnification
Black and white images
Sample must be in a vacuum, and therefore non-living
How do transmission electron microscopes work?
Extremely thin specimens are stained and placed in a vacuum . An electron gun produces a beam of electrons that pass through the specimen. Some parts absorb the electrons and appear dark. The image is produced in 2D and shows detailed images on the internal structure of cells
How do scanning electron microscopes work?
The specimens do not need to be thin, as the electrons are not transmitting through. Instead, the electrons are beamed onto the surface and the electrons are scattered in different ways depending on the contours. This produces a 3D image
What is the formula for magnification?
magnification = image size/actual size
How do you calibrate the eyepiece in optical microscopes?
Step 1- Line up the stage micrometer and eyepiece graticule whilst looking through the eyepiece
Step 2- Count how many divisions on the eyepiece graticule fit into 1 division on the micrometer scale
Step 3- Each division on the micrometer is 10 micrometers so this can be used to calculate what one division on the eyepiece graticule is at that current magnification
What is cell fractionation?
Used to isolate different organelles so they can be studied. This enables individual organelle structures and functions to be studied. The cells must be prepared in a cold, isotonic, and buffered solution
Explain when cells are being fractionated they must be prepared in a cold, isotonic and buffered solution.
Cold- to reduce enzyme activity - reduces damage to organelles
Isotonic - must be the same water potential to prevent osmosis as this could cause organelles to shrivel or burst
Buffered- the solution has a maintained pH to prevent damage to organelles
What are the 2 steps to cell fractionation?
Homogenisation - The cell must be broken down using a blender.Blended in a cold, isotonic and buffered solution. This solution is filtered to remove large cell debris
Ultracentrifugation - The filter is spun at different speeds in a centrifuge.
Organelles separate according to their densities
Explain differential centrifugation
The centrifuge spins and the centrifugal forces cause pellets of the most dense organelles to form at the bottom
The centrifuge is first spun at a low speed and the process is repeated at increasingly faster speeds
Each time the supernatant (liquid) is removed, leaving behind a pellet of organelles
The supernatant is then spun again to remove the next pellet of organelles
What is diffusion?
The net movement of particles from an area of higher concentration to an area of lower concentration
In CSM particles that pass through must be lipid-soluble
Passive process - requires no input of energy in the form of ATP