Topic 1- Proteins Flashcards
What are proteins?
Proteins are biological molecules that are composed of carbon, hydrogen, oxygen and nitrogen.
What are the monomers of proteins?
Amino acids.
What bond is formed via a condensation reaction of 2 amino acids?
Peptide bond.
What do you form when two amino acids join together?
A condensation reaction takes place forming a dipeptide.
What do you form when more than two amino acids join together?
A condensation reaction takes place between the amine group of one amino acid and the carboxyl group of another forming a polypeptide.
Describe the structure of an amino acid.
- Amine Group (H2N)
- Carboxyl Group (COOH)
- A variable carbon-containing group (R)
- A hydrogen (H)
How many different amino acids can you get?
There are 20 different amino acids, each determined by their different R groups.
What does a functional protein contain?
One or more polypeptides.
What are the 4 structures that proteins are called?
Primary
Secondary
Tertiary
Quaternary
What is meant by the primary structure of proteins?
The sequence of amino acids in the polypeptide chain.
What is meant by the secondary structure of proteins?
The way in which the chain of amino acids of the polypeptides of a protein is folded. Hydrogen bonds form between the amino acids, to form 3D shapes like an alpha helix or beta pleated sheet.
What is meant by the tertiary structure of proteins?
Further folding of a whole polypeptide chain in a precise way. The tertiary structure is held together by bonds between the R groups of the amino acids in the protein, and so depends on what the sequence of amino acids is.
There are three kinds of bonds involved:
1. Hydrogen bonds, which are weak.
2. Ionic bonds between R-groups with positive or negative charges, which are quite strong.
3. Disulphide bridges - covalent S-S bonds between two cysteine amino acids, which are strong.
What is meant by the quaternary structure of proteins?
More than one polypeptide chains linked together and held by bonds.
What determines the function of a protein?
The shape of the protein.
Describe the test for proteins.
The test for proteins is known as the biuret test
The steps are:
- Add the test sample to a test tube.
- Then add sodium hydroxide solution as the test solution needs to be alkaline.
- Then add a few drops of copper(II) sulfate solution.
- If the solution turns from blue to purple, then it indicates that protein is present.