Topic 1 - Biomolecules Flashcards
What is the chemical composition of a bacterial cell?
Include:
- How much that component contributes to the overall weight (%)
- Number of species
- how amazing Ambre is!
Important things to note:
- Ions mentioned in the photo are the main ones
- Large difference in diversity between the macromolecules.
Definition of a metabolite?
A substance that is a product of metabolic action or that is involved in a metabolic process.
Definition of a cofactor?
They are inorganic or organic chemicals that assist enzymes during catalysis of reactions.
What are the main elements involved in living systems?
The main bulk of biological elements:
Ions - Na, Mg, K, Ca, Cl
Main elements organic molecules - C, H, N, O
Extra elements - P, S
What are trace elements involved in living systems?
Trace elements used for specialized properties/reactions.
- Transition metals (V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Mo)
- Extra –> Se and I
What are the three main types of chemical reactions in living systems?
- Protonation/deprotonation –> Coming and going of Hydrogen ions
- Condensation/Hydrolysis –> H2O coming and going
- Oxidation/reduction –> e- / H+ coming and going
Note –> Movement of e- and H+ are equivalent.
Explain what happens during protonation/deprotonation reactions?
(Acid/Base chemistry)
Examples - Carboxyl/amine group/phosphoric acid
Note -> Acids donates H+ / Base accepts H+
Explain what happens during condensation/hydrolysis reactions?
Examples - Ester/Phosphate ester/Phosphoanhydride
Explain what happens during Oxidation/reduction reactions?
Example - Ethane –> ethene
What is a phosphodiester bond?
Basically….
R — O — P — O — R
How to approach bonding questions with large molecules?
Example –> Practise drawing the deprotonation/condensation of phosphates in ATP.
With large molecules –> find the r-group that isn’t involved in bonding and ignore it.
For example:
Condensation and deprotonation of phosphate groups in ATP –> Ignore the Adenosine and Ribose molecule.
Molecules bonding drawing practise.
Reduction of nicotinamide-adenine dinucleotide (NAD+) to NADH + H+
- Draw condensation reaction to form nicotinic acid
- Draw reduction of nicotinic acid.
What do the law of thermodynamics help us with?
They determine the conditions under which specific processes can or cannot take place.
What is the 1st law of thermodynamics?
1st law of thermodynamics –> States that the total energy of a system and its surroundings is constant.
Hence, the energy content of the universe is constant (cannot be created or destroyed). However, it can take different forms –> Heat a form of kinetic energy or potential energy is released during the occurrence of a process.
What is the 2nd law of thermodynamics?
2nd law of thermodynamics –> States that the total entropy of a system plus that of its surroundings always increases.
Sometimes the 2nd law is contradicted –> i.e. plants use CO2 + other nutrients to form leaves. However, in such cases entropy is decreased locally, whereas, entropy elsewhere increases.
Additionally –> local decreases often results in a heat release –> increases entropy of surroundings.
What are the two conditions needed for a chemical reaction?
- Reaction needs a mechanism
- Follow the law of thermodynamics.
What is chemical potential energy?
Normally during reactions:
Chemical energy —> Heat
This chemical energy is stored as Chemical potential energy.
Chemical potential energy –> Energy stored in the chemical bonds of a substance. It is represented by mu (µ).
What is the formula to calculate Gibbs free energy from the chemical potential energy of molecules?
Given the following reaction:
A + B –> C + D
We can say that….
(µc + µD) - (µA + µB) = ΔG
Basically…
The change in µ of products minus change in µ of reactants.
What is Gibbs free energy?
Gibbs free energy is an accounting tool that keeps track of both the entropy of the system (directly) and the entropy of the surroundings (in terms of the heat released from the system).
How can we calculate Delta G from the equilibrium constant?
For a given equilibrium reaction:
A + B <—> C + D
The following equilibrium constant is obtained:
Keq = ((C)(D))/((A)(B))
When the constant and the following equation are used we can calculate Delta G.
ΔG = -RTLn(k)
As we can see, this equation links ΔG and K.
What are the different results for the ΔG = -RTLnK equation?
- K = 1 –> ΔG = 0
- K > 1 –> ΔG = Negative –> favours products
- K < 1 –> ΔG = Positive –> favour reactants
Hence….
More negative ΔG is —> more the reaction is favoured –> as more product is formed.