topic 1 Flashcards
3 monosaccharides
glucose fructose galactose
starch
energy store in plant cells
polysach of alpha glucose
amylose 1-4 glycosidic bonds so it is unbranched
amylopectin 1,4,1,6 branched
helical so compact for storage
large so cant leave the cell
insoluble in water so water potential of cell not affected
glycogen
energy store in animal cells and is a polysaccharide of alpha glucose
1-4 2-6 glycosidic bonds so branched so its compact and can fit lots of molecules in a small area
branched also means more ends for hydrolysis so release glucose for respiration to make ATP for energy release large and insoluble so can’t leave cell and as its insoluble in water wp of cell unaffected
cellulose
strength and structural support to plant cells and algal cell walls
polysach of B – 1,4 glycosidic bonds so unbranched chains that link parallel by hydrogen bonds forming microfibrils every other b glucose molecule is inverted in long straight unbranched chain and many hydrogen bonds link parallel strands to form microfibrils hydrogen bonds are also strong in high numbers so provide strength
how to find quantity of sugar in a solution
make sugar dilutions of known concentrations heat volume of each sample with set volume of benedicts for same time and use colorimeter to measure absorbance of each known concentration plot calibration curve conc on x and absorbance on y and draw line of best fit then repeat benedicts with unknown samples
Triglycerides
1 glycerol 3 FA 3 ester bonds there is a high ratio of C-H:C in HC chain so used in respiration to release more energy and insoluble in water so no effect on WP
competitive inhibitor
as conc of comp inhibitor increases ROR decreases as it is a similar shape to the substrate it blocks the active sites so substate can’t bind so fewer E-SC
non competitive inhibitor
as concentration of it increases, reaction rate decreases
it binds to the allosteric site changing the enzyme tertiary structure so active site is no longer complementary to substrate so substrate can’t bind so fewer E-SC – this is permanent so increasing substrate conc has no effect
dna
hold genetic info for polypeptides
rna
transfer genetic info from dna to ribosome
How to nucleotides join to form polypeptides
condensation reaction between phosphate group and deoxyribose forming phosphodiester bonds
dna structure
– polymer of nucleotides and each nucleotide is formed from a phosphate group a nitrogen containing base and phosphodiester bonds join adjacent nucleotides.2 polynuc chains held together by h bonds between specific comp base pairs and it is a double helix
how does dna structure elated to function
two strands so both Can act as a template for semi conservative replication
hydrogen bonds between bases are weak so strands can be separated for replication
comp base pairing so accurate replication
many hydrogen bonds between bases so strong stable molecule
long molecule so it can store lots of genetic info
semi conservative replication
dna helicase breaks hydrogen bonds between comp base pairs
unwinding the double helix both strands act s a template
free dna nucleotides attracted to exposed bases and join by specific comp base pairing
hydrogen bonds form between a and t and g and c
dna polymerase joins adjacent nucleotides on new strand via condensation
forming phosphodiester bonds
why does DNA polymerase move in opposite directions along dna strands
dna has antiparallel strands
so shape of nucleotides on two ends different
dna polymerase is an enzyme with specific shaped active site
so can only bind to substrate with comp shape