Tissue & Cell Culture

Question 1

Cell Culture

Answer 1

Culturing of dissociated cells outside of organism and eukaryotic tissues
Use growth medium

Question 2

Contact Inhibition

Answer 2

Cells stop dividing/growing when they come in contact with each other

Question 3

Passaging

Answer 3

Replacing cells to avoid overcrowding

Question 4

Freezing & Thawing Cells

Answer 4

Cryoprotectant - prevent ice crystals (DMSO)
Controlled - rapid thawing to minimize cell damage

Question 5

Mycoplasma

Answer 5

Smallest bacteria cell
Resistant to many antibiotics

Question 6

Types of Cells

Answer 6

Primary - normal tissue
Cell strains - limited growth/division
Cell lines - grow indefinitely
HeLa - immortalized

Question 7

Induced Pluripotent Stem Cells (iPSCs)

Answer 7

From reprogramming animal and human differentiated cells (skin and blood)

Question 8

Embryonic Stem Cells

Answer 8

Pluripotent cells which give rise to all somatic cell types in the embryo (understand development of specialized cells and establishment of organ structures)