Electrophysiology Flashcards
Week 2 - three lectures
Resting Membrane Potential
-60 to -80 mV inside neuron
KCC - potassium + chloride co-transporter
Na+, K+, ATPase - sodium/potassium exchanger
Negative Resting Membrane Potential
-70 mV
Postsynaptic Potenial
Small variable changes in membrane potential
1-40 mV
Action Potential
Large, fast, travel long distances, all or nothing
<100 mV
ATP
Adenosine Triphosphate
Extracellular energy molecule
Excitatory Postsynaptic Potentials (EPSP)
Ion channels that depolarise neurons
Inhibitory Postsynaptic Potentials (IPSP)
Ion channels that hyperpolarise neurons
Extracellular Recording (ER)
Field potential
Whole nerve activity
Multi-unit activity
Single-unit activity
Multi-electrode arrays (MEAs)
Intracellular Recordings (IR)
Activity within single cell
Sharp electrodes
Patch suction
Current / Voltage Clamp
Single Channel Recording (SCR)
Activity of single ion channel
Patch clamp
Field Potential
Electric potential in extracellular space around neurons
Nerve
Bundle of axons
Patch-Clamp Technique
Record electrical activity:
On-cell
Inside-out
Whole-cell
Outside-out
Perforated
Application of Techniques
Humans (in vivo) - ER (as part of treatment)
Non-humans (in vivo) - ER, IR, SCR
Tissue (in vitro) - ER, IR, SCR
Electrophysiology
Record electrical activity of whole brain tissue, single neuron or single ion channel
Understand nervous system
Summary of biological electrical activity
