Thrombin Clotting Time (F) Flashcards
Thrombin clotting time is also called as what?
Thrombin time
What are the purposes of TCT?
1) Tests the efficiency of the last part of common pathway in vitro
2) Monitors the effects of dabigatran
3) Determines the presence of unfractionated heparin, paraproteins and fibrin degradation products
4) Indicator of afibrinogenemia and dysfibrinogenemia
What is the simplified process of TT?
1) PPP + thrombin (bovine or human)
2) Record clotting time
What is the simplified process of TT?
1) PPP + thrombin (bovine or human)
2) Record clotting time
What is the simplified process of TT?
1) PPP + thrombin (bovine or human)
2) Record clotting time
What is the simplified process of TT?
1) PPP + thrombin (bovine or human)
2) Record clotting time
What is the simplified process of TT?
1) PPP + thrombin (bovine or human)
2) Record clotting time
What is the simplified process of TT?
1) PPP + thrombin (bovine or human)
2) Record clotting time
What is the simplified process of TT?
1) PPP + thrombin (bovine or human)
2) Record clotting time
What is the simplified process of TT?
1) PPP + thrombin (bovine or human)
2) Record clotting time
What is the composition of TCT rgnt?
Exogenous thrombin + ionized calcium
What is the purpose of exogenous thrombin?
It substitutes for the factor IIa for common pathway in vivo
What is the characteristic of exogenous thrombin?
It could be recombinant (human or bovine source)
What is the characteristic of TCT rgnt?
It is often in lyophilized form
What are the diff methods of TCT?
1) Manual method
2) Semi-automated method
3) Automated method
What are the characteristics (in terms of process) of manual method?
1) Uses test tubes
2) Uses H2O bath
3) Manual rgnt aspiration
4) Manual sx aspiration
5) Manual timer
6) Manual incubation
7) Does not use a magnetic stir bar
8) Detects visible clot formation using our own eyes
What are the characteristics (in terms of process) of manual method?
1) Uses test tubes
2) Uses H2O bath
3) Manual rgnt aspiration
4) Manual sx aspiration
5) Manual timer
6) Manual incubation
7) Does not use a magnetic stir bar
8) Detects visible clot formation using our own eyes
What are the characteristics (in terms of process) of manual method?
1) Uses test tubes
2) Uses H2O bath
3) Manual rgnt aspiration
4) Manual sx aspiration
5) Manual timer
6) Manual incubation
7) Does not use a magnetic stir bar
8) Detects visible clot formation using our own eyes
What are the characteristics (in terms of process) of manual method?
1) Uses test tubes
2) Uses H2O bath
3) Manual rgnt aspiration
4) Manual sx aspiration
5) Manual timer
6) Manual incubation
7) Does not use a magnetic stir bar
8) Detects visible clot formation using our own eyes
What is the process (or steps) of manual method?
1) Reconstitute TCT rgnt by adding TCT diluent or distilled H2O into the rgnt bottle. Incubate at 37 DC
2) Transfer 100 uL of citrated PPP into a tube / cuvette. Incubate this aliquot at 37 DC for 3 - 10 mins
3) Add 200 uL of warmed TCT rgnt directly to the warmed citrated PPP
4) Start timer. Record the time until visible clot formation is observed
What are the characteristics (in terms of process) of semi-automated method?
1) Uses large cuvettes
2) Uses digital dry bath
3) Manual rgnt aspiration
4) Manual sx aspiration
5) Automated timer
6) Manual incubation
7) Uses a magnetic stir bar
8) Detects visible clot formation using optical sensors
What are the characteristics (in terms of process) of automated method?
1) Uses small cuvettes
2) Uses rxn wells
3) Automated rgnt aspiration
4) Automated sx aspiration
5) Automated timer
6) Automated incubation
7) Does not use a magnetic stir bar
8) Detects visible clot formation using optical or electromechanical sensors
What are the results in secs (w/ corresponding interpretations) of TCT?
1) < or equal to 21 secs: normal
2) > 21 secs: prolonged
