THREADGILL LECTURES

Question 1

Gives 4 therapeutic applications of PARP-1 inhibition

Answer 1

1) potentiation of radiotherapy and cytotoxic chemotherapy of cancer
2) reduction of organ damage following ischaemia/reperfusion injury
3) anti inflammatory
4) anti-retro viral

Question 2

What is PARP essential for?

Answer 2

Repair of nucleotide excision repair
Single stranded break repair
And to some extent double stranded breaks

Question 3

What is O6-alkylguanineO6-alkyl transferase

Answer 3

MGMT- suicide enzyme that repairs alkylation/methylation damage at O6 guanine

Question 4

Name two MGMT inhibitors

Answer 4

O6-benzylguanine

Lomeguatrib- patrin-2

Question 5

What happens to MGMT after its repaired the DNA

Answer 5

Adds ubiquitin the hydrolysed (suicide enzyme)

Question 6

What is PARP-1 structure made up of

Answer 6

Binding domain
Auto modification domain
Catalytic domain

Question 7

What’s contained on the binding domain of PARP-1?

Answer 7

2 (3) zinc fingers needed to insert into DNA

Question 8

How many KDa is MGMT

Answer 8

24 - small and soluble

Question 9

How many KDa is PARP-1?

Answer 9

116KDa