THICK AND THIN

Question 1

Purpose OF THIN FILM & THICK FILM

Answer 1

THICK: To search for malaria parasites
THIN: To confirm the parasite species

Question 2

Is important in making the definitive diagnosis and remains as the gold standard

Answer 2

Microscopic identification of malarial parasites in thick and thin blood smears

Question 3

Name the (4) medically important malarial species

Answer 3

Plasmodium falciparum
Plasmodium vivax
Plasmodium ovale
Plasmodium malariae

Question 4

Most common method in staying blood films with giemsa stain

Answer 4

Rapid (10%) method

Question 5

This method is ideal for staining blood films from surveys or research work

Answer 5

Slow (3%) Method

Question 6

How many slides can be stained at a time

Rapid (10%) Method - _____
Slow (3%) Method - ______

Answer 6

1-15 slides
20 or more slides

Question 7

Composition of Giemsa staining and color

Answer 7

Mixture of eosin, which stains parasite chromatin

Stippling shades of red or pink and methylene blue (stains parasite cytoplasm blue)

Question 8

What is Giemsa staining

Answer 8

An alcohol-based Romanowsky stain

Question 9

Duration of the immersion of Giemsa staining in

A. Rapid (10%) Method
B. Slow (3%) Method

Answer 9

A. 8-10 minutes
B. 45-60 minutes

Question 10

Characteristics of Good Peripheral Smear

Answer 10

• Has a feathery-edge
• Not too short
• Not too long

Question 11

It has been identified by the WHO as one of the three major infectious diseases (along with HIV/AIDS, tuberculosis)

Answer 11

Malaria

Question 12

How is malaria transmitted?

Answer 12

From one person to another by the bite of a female Anopheles mosquito

Question 13

What is CLSI’s recommended puncture/incision site for adults and children over 1 year old

Answer 13

Palmar surface of the distal or end segment of the middle or ring finger of the nondominant hand