DFS, Iodine Staining and KOH Wet Mount Flashcards
Fill in the blanks or its pair
LIQUID = ________
______ = Cysts
______ = both cysts and trophozoites
ANY CONSISTENCY = ______
Protozoan trophozoites
Formed
Soft
Helminth eggs and larvae
What is a properly prepared smear?
Allow ordinary newspaper print to be barely visible through the preparation
What is the use of saline mount and iodine mount in D’ Antoni’s Method?
Saline mount: Recovery of trophozoites
Iodine mount: Destroys trophozoites
What is the acceptable amount of stool required for a parasite study? What is it often referred to as?
2 to 5 g; size of a walnut
The most common procedure performed is the examination of a stool specimen for ______
Ova and parasites
All stool samples should be collected in a _______ container like a ________, _______(1/2 point) or ______
Suitable, clean, wide-mouthed
Plastic container with a tight-fitting lid
Waxed cardboard box or matchbox
All purpose fixative, buffered with sodium phosphate to preserve the morphological characteristics
Formalin
The recommended fixative:specimen ratio for stool preservatives
3:1
STOOL
5% formalin concentration is recommended for _______
__________ is recommended for helminth eggs and larvae
Protozoan cysts
10% formalin concentration
Used to preserve fresh stool/fresh fecal specimen in preparation for staining stool smears
Schaudinn’s solution
Viable alternative to the use of PVA and Schauddin fixative
Sodium acetate or Acetic acid formalin (SAF)
(3) primary advantages of the use of formalin in stool preservatives
Easy to prepare
Preserves specimens for up to several years
Long shelf life
Liquid specimens of stool are examined within ______; semi-formed specimens of stool within ______
30 min; 1 hour
Why should wet mounts in stool be examined immediately after preparation?
To avoid evaporation of liquid
What is the principle of D’ Antoni’s method?
To detect protozoan cyst
Purpose of D’ Antoni’s method
Most useful for recognition of cysts stages
Iodine kills and distorts the trophozoites
What should contain the following information of the every specimen?
Patient’s name
Age/Sex
Identification number
Physician’s name
Date and Time the specimen was collected
Requested procedure
Presumptive Diagnosis
Prior infections
Travel history
How much is required in a routine stool examination?
Thumb-sized of formed stool or about 5-6 tablespoons of watery stool
What are fixatives?
Substances that preserver the morphology of protozoan and prevent further development of certain helminth eggs and larvae
How long must the specimen (stool) be fixed in the preservative before processing begins?
30 minutes
Blood and mucus in soft or watery stools may possibly yield the presence of _____
Trophozoites
FILL IN THE BLANKS
Routine fecalysis (importance)
To detect the presence of ______
For the detection of ____
Used as a clue in ______ diagnosis
For the detection of excessive _______ (_____)
For the detection of evidence of malfunction of some parts of ____, _____, and ______
Intestinal parasite
Gastrointestinal tract bleeding
Medical and surgical
Fats in stool; steatorrhea
GIT, liver, pancreas
