Thermal Preservation of Foods Flashcards

1
Q

What are the three categories of thermal preservation of foods?

A

blanching

pasteurization

commercial sterilization

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2
Q

What is blanching?

A

Blanching is a form of thermal processing applied mainly to vegetables and some fruit by exposing them to heated or boiling water or even culinary steam for a short period of time.

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3
Q

What is blanching as a food processing operation designed to do?

A
  • inactivate enzymes in plant tissues so that enzymatic degradation does not occur in the interval between packaging and thermal processing or during frozen storage or in the early stages of food dehydration and after reconstitution of dehydrated plant foods.
  • wilt vegetable products to enable packing of the products into containers so that proper fill weights can be achieved.
  • drive off inter- and intracellular oxygen and other gases from plant tissues so that containers are not deformed by excessively high internal pressures due to expanding gases within the container and to permit formation of a vacuum in the container after thermal processing
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4
Q

What is pasteurization?

A

Pasteurization is a thermal process that involves using temperatures of at least 72°C for 15 seconds (high temperature short time or HTST process), prior to packaging.

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5
Q

What is the basis for preservation by pasteurization?

A

To inactivate pathogenic (disease causing) bacteria and viruses in low acid food products such as milk.

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6
Q

What food products are pasteurized?

A

Acid food products (pH < 4.6) are mainly pasteurized to inactivate spoilage-causing microorganisms.

Pathogenic microorganisms cannot grow and do not survive very well in acid foods such as citrus juices or apple juice.

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7
Q

Can microorganisms survive pasteurization?

A

In low-acid and acid foods, many spoilage-causing microorganisms can still survive typical pasteurization process conditions:

  • In milk, the proteolytic and lipolytic bacteria are more heat resistant and can survive the pasteurization process. This explains why the typical spoilage pattern of pasteurized milk reflects the proteolytic (protein degradation) and lipolytic (lipid degradation) action of the psychrotrophic, spoilage-causing bacteria.
  • Because pasteurization does not kill all the psychrotrophic spoilage-causing bacteria in milk, pasteurized milk must be refrigerated to maintain shelf life quality.
  • The durable life date on milk containers reflects the storage life that can be expected when milk is held at 4 °C or lower.
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8
Q

What bacteria in milk can survive the pasteurization process?

A

In milk, the proteolytic and lipolytic bacteria are more heat resistant and can survive the pasteurization process.

This explains why the typical spoilage pattern of pasteurized milk reflects the proteolytic (protein degradation) and lipolytic (lipid degradation) action of the psychrotrophic, spoilage-causing bacteria.

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9
Q

What bacteria (classification) survives pasteurization?

A

Psychrotrophic spoilage-causing bacteria

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10
Q

What is commercial sterilization?

A

This thermal process involves heating the food with a minimum treatment of 121°C moist heat for 15 minutes. The process usually involves pre-sealing the food in containers prior to heating (also known as “canning”). Other forms of CS involve heating the food before it is aseptically packaged (UHT-Aseptic packaging).

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11
Q

What is the basis for commercial sterilization?

A

The basis for preservation by CS is to destroy both spoilage and disease causing microorganisms in low-acid and acid foods, thus rendering the food “commercially sterile”.

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12
Q

What does commercially sterile as described in the Food Regulations (Division 27) of the Food and Drugs Act of Canada mean?

A

The condition obtained in a food that has been processed by the application of heat, alone or in combination with other treatments, to render the food free from viable forms of microorganisms, including spores, capable of growing in the food at temperatures at which the food is designed normally to be held during distribution and storage.

Therefore, commercially sterilization involves the destruction of spoilage-causing and disease-causing microorganisms*

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13
Q

Commercially sterile foods may contain small numbers of extremely thermophilic bacteria spores.

True or false?

A

True

Commercially sterile foods may contain small numbers of extremely thermophilic bacteria spores; however, the spores cannot germinate and produce actively growing cells at room temperature, nor would they cause disease.

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14
Q

If a can of food is being sterilized, what heat treatment must each food particle receive?

A

Today, if a can of food is being sterilized, each food particle must receive the heat treatment (e.g. 121°C for 15 min).

When food is placed in a can, the heat treatment will change since heat transfer to the food takes place at a slower rate. Depending on the size of the can, the time to achieve sterility could be several hours.

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15
Q

What is the shelf-life of a commercially sterile product?

A

Most commercially sterile products have a shelf life of 2 years or more.

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16
Q

What is the basis for ultra-high temperature processing?

A

The basis of UHT and aseptic packaging is the application of “ultra high temperature” (heat) to food before packaging, then filling the food into pre-sterilized containers in a sterile atmosphere.

This process will render the food shelf stable or commercially sterile without the need for refrigeration.

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17
Q

What is ultra-high temperature aseptic packaging?

A

UHT- Aseptic packaging is a relatively new development whereby food can be heated to 140-150°C very rapidly by direct injection of steam, held at that temperature for short period of time (e.g. 4-6 seconds) and then cooled, in a vacuum chamber to flash off the water added in the form of condensed steam.

This is carried out as a continuous flow operation.

The decrease in processing time due to the higher temperature, and the minimal come-up time and cool-down time leads to a higher quality product.

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18
Q

How are UHT processed foods packaged?

A

The UHT processed food is aseptically packaged into pre-sterilized containers.

These are usually cartons made from laminated plastic, aluminum and paper, which are chemically sterilized with a combination of hydrogen peroxide and heat, and then filled in the same piece of equipment which is housed in a sterile environment.

There are other forms of packaging that can also be used in aseptic UHT processing: plastic cans, flexible pouches, thermoformed plastic containers, bag-in-box, and bulk totes.

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19
Q

What is the shelf-life of UHT-aseptically packaged foods?

A

UHT-aseptically packaged products have a shelf life of 6 months or more, without refrigeration. It depends on the type of packaging being used.

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20
Q

Give examples of food products processed with UHT.

A
  • liquid products: milk, juices, cream, yogurt, wine, salad dressings
  • semi-liquid/solid products: baby foods; tomato products, fruits and vegetable juices, soups.
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21
Q

UHT processed milk and juices contain added agents to provide the long storage life at ambient temperature in the laminated cartons.

True or false?

A

False.

Contrary to popular opinion, UHT processed milk and juices do not contain added agents to provide the long storage life at ambient temperature in the laminated cartons.

The products are preserved solely through the application of heat.

It is critical that the sterilized products are transferred to packaging equipment under aseptic conditions, to avoid contamination after thermal processing.

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22
Q

All products that are UHT treated are aseptically packaged.

True or false?

A

False

Many products that are UHT treated are not necessarily aseptically packaged.

This gives them the “advantage” of a longer shelf life at refrigeration temperatures compared to conventional pasteurized (HTST) products.

However, this does not produce a shelf-stable product at ambient temperatures due to the possibility of post-processing recontamination.

23
Q

What are the main considerations in selecting the required temperature-time conditions for thermal processing?

A

The intensity of the heat treatment employed for a particular food preservation application depends upon a number of factors:

  • What is the objective or purpose? (blanching or pasteurization or commercial sterilization)
  • Are there additional preservation steps? (is it combined with other preservation methods?)
  • What are the physical, chemical properties of the food? (Type of food)
  • What is the heat resistance of microorganisms in the food?
24
Q

How may the shelf-life of foods that will be consumed within a short period of time after processing be extended?

A

By a combination of pasteurization and refrigerated storage (used for pasteurized milk and for pasteurized, vacuum packaged, cured meats).

25
Q

What are the thermal processed applied to foods governed by?

A

The heat resistance of the microorganisms in the food.

26
Q

What does longer storage times at ambient temperatures in evacuated sealed containes require?

A

The use of commercial sterilization.

27
Q

What is the time-temperature combination required for pasteurization and commercial sterilization determined by?

A

The most heat-resistant disease-causing and spoilage-causing microorganisms in the particular food commodity.

28
Q

What is the type of thermal processing operation and the rate of heat penetration into the slowest heating portion of the food within a particular container governed by?

A

The food’s physical properties (solid vs. liquid, or solid particles suspended in a liquid) and chemical properties (pH, fat content, presence or absence of heat-inducible thickening agents, food components that have protective or antagonistic effects on the thermal resistance of microorganisms).

29
Q

How should thermal preservation processed be designed?

A

It is imperative that thermal preservation processes be designed so that the slowest heating portion of the food commodity receives the specified time-temperature thermal treatment to minimize risks of illness and/or post-processing spoilage.

30
Q

What is the microorganism of most concern in low acid foods that are to be thermally processed and vacuum sealed within gas-tight containers?

A

The microorganism of most concern is Clostridium botulinum.

The habitat of Clostridium botulinum can be soil (agricultural and forest), water (fresh, brackish and marine) and mud (fresh water and salt water).

As a consequence, all foods of agricultural and fisheries origin must be considered as being potentially contaminated with Clostridium botulinum spores.

31
Q

What is the habitat of Clostridium botulinum?

A

Soil (agricultural and forest), water (fresh, brackish and marine) and mud (fresh water and salt water).

As a consequence, all foods of agricultural and fisheries origin must be considered as being potentially contaminated with Clostridium botulinum spores.

32
Q

What do low acid foods that are to be packaged and stored under anaerobic conditions require?

A

A specifically designed thermal processing treatment to ensure the destruction of any Clostridium botulinum spores.

This will provide a large margin of safety.

Actively growing vegetative Clostridium botulinum cells produce a very potent neurotoxin.

33
Q

How can we determine if C. botulinum spores have been destroyed?

A

To determine the thermal resistance of heat-resistant spores in foods, “Inoculated pack studies” are carried out using a non-pathogenic spore-forming bacterium, Clostridium sporogenes PA3679 (a putrefactive anaerobe).

Since PA3679 spores are more heat resistant than those of Clostridium botulinum spores, a process designed to kill PA3679 spores will definitely kill Clostridium botulinum spores with a wide margin of safety.

34
Q

Describe the graph of microbial death during thermal processing.

A

When microorganisms (bacteria, such as vegetative cells and spores, moulds, viruses and yeasts) are exposed to high temperatures capable of causing death of the organisms, one observes that the population is not killed instantaneously.

We see that microbial death during thermal processing follows a logarithmic order.

This means that bacteria are killed by heat at a rate that is nearly proportional to the number present in the system being heated.

35
Q

What does one logarithmic cycle represent?

A

The survivor curve or thermal death rate curve depicts the logarithmic order of death.

You will note that the time taken to traverse one logarithmic cycle represents the time, at a constant temperature, required to kill 90% of a microbial population.

The time required to kill 90% of the microbial population exposed to a specific temperature is defined as the decimal reduction time or D-value

A sample calculation of the decimal reduction time follows:

If we were to start out with a population of 105 (=100,000) bacterial cells in a unit volume or mass of food at time ‘A’, only 104 (=10,000) cells would survive after one logarithmic cycle on the graph was traversed (time ‘B’).

36
Q

How do you calculate the D value?

What is the D value useful for?

A

Assume that time ‘A’ is 5 minutes and that time ‘B’ is 10 minutes.

During the time (10-5 = 5 minutes) that the survivor curve traversed one logarithmic cycle, 90% of the microorganisms were killed (10% survived) by the exposure to the constant temperature.

The time taken to kill 90% of the microbial population was 5 minutes.

The D-value at that particular temperature was 5 minutes.

D-value is a useful index of the heat resistance of a particular microorganism to the killing effects of heat at a particular temperature.

However, also note that D-values apply to a specific microorganism under a specified set of conditions (temperature, type of food).

37
Q

What does the magnitude of the D-value depend on?

A

If the temperature is increased, the D-value would decrease because the rate of microbial death would increase.

The magnitude of the D-value depends on how the constituents of the food affect the sensitivity of the microorganism to the killing effects of heat.

38
Q

What is a thermal death rate curve?

A

It describes the rate of death of a particular microorganism under a specified set of conditions.

A thermal death time curve can be constructed from a number of thermal death rate curves by exposing the microorganism to a variety of temperatures and determining the decimal reduction time at each temperature.

Note: vegetative bacteria cells have a much lower heat resistance than spores.

39
Q

What is the F-value and what does it indicate?

A

The number of minutes at a specific temperature required to destroy a specified number of organisms having a specific z-value, a mathematically calculated number that describes the total lethal effects of the process at the slowest heating point in a food container.

The standard reference temperature is generally selected as 121.1°C (250 °F), and the relative time (in minutes) required to sterilize any selected organism at 121°C is known as the F-value of that organism.

The F-value is the equivalent of all heat considered with respect to its capacity to destroy spores or vegetative cells of a particular microorganism.

In other words, it is a measure of “lethality” or the capacity of the heat treatment to sterilize.

40
Q

Vegetative bacteria cells have a much higher heat resistance than spores

True or false?

A

False.

Vegetative bacteria cells have a much lower heat resistance than spores.

41
Q

What do the z-values and F-values indicate?

A

These two terms characterize a thermal death time curve.

42
Q

What is the z-value and what does it indicate?

A

The number of degrees required for a specific thermal death time curve to pass through one log cycle.

Different microorganisms in a given food will have different z-values.

Similarly, a given microorganism will have different z-values in different foods.

The z-value indicates the resistance of a microbial population to changing temperature.

43
Q

As the spore population in a food system is increased, the total time required at a particular temperature to kill ALL the spores increases.

True or false?

A

True.

44
Q

What margin of safety is applied for low acid foods?

A

Typically, for low acid foods (pH greater than 4.6) a margin of safety of 12D is applied.

45
Q

What margin of safety is applied for acid foods?

A

Typically, a 5D thermal process is usually used for acid foods.

46
Q

What does ‘margin of safety’ refer to?

A

Inherent in the thermal processing of foods is the concept of a margin of safety which refers to the probability that a container of food could still contain a viable spore of Clostridium botulinum after the completion of the thermal processing.

47
Q

What are factors that affect the heat transfer characteristics of a food?

A
  • the consistency of the food (liquid -viscous or non viscous- or solid)
  • the chemical composition of the food.
  • other factors that are important are the container size, shape and composition.
48
Q

What is the cold point?

A

the slowest heating part of the food

49
Q

What is a retort and what is its function?

A

Foods that are thermally processed after being packed in containers (such as metal cans, glass bottles, plastic pouches) are exposed to an environment of pressurized steam within a vessel called a retort.

A retort operates very much on the same principles as the pressure cooker or pressure canner with which you may be more familiar. Packages of foods are placed in the retort after which the retort is sealed and the air within is vented by purging the retort with steam. Once the retort is properly vented, the steam pressure inside is increased to achieve the desired processing temperature.

50
Q

How does a retort heat a solid food?

A

If the food is a solid (salmon, for example), heat energy is transferred by conduction

51
Q

How does a retort heat non-viscous liquids?

A

Foods that are non-viscous liquids (canned evaporated milk, for example) heat by convection.

52
Q

How does a retort heat foods that are a combination of solid and liquid components?

A

Foods that are a combination of solids and liquid components heat up by a combination of convection and conduction heating.

53
Q

Why must food processing companies be very careful when they reformulate foods preserved by thermal processing?

A

Sugars, oils, fats and salt can have the effect of protecting spores and vegetative cells from the killing effects of heat, thus requiring use of longer exposure times or higher temperatures for processing those foods exhibiting these protective effects.

Certain spices may have antimicrobial activity and change the resistance of microorganisms and spores to the killing effects of high temperatures (D and z values are decreased).

54
Q

What are the most common ways to get botulism?

A

“improperly prepared low-acid, home-canned foods (like asparagus, beets, green beans, mushrooms, peppers); improperly smoked fish; improperly prepared raw marine mammal meat (like whale, walrus, seal);non-refrigerated storage of low-acid fruit juices (like carrot juice); baked potatoes stored in aluminium foil” are the most common ways to get botulism.