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Biochem Exam 3 > Thermal DNA Denaturation > Flashcards

Thermal DNA Denaturation Flashcards

1
Q

The midpoint of melting (Tm) depends on

A

Base composition

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2
Q

High CG

A

Increases Tm= more hydrogen bonds

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3
Q

Tm depends on

A

Dna length

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4
Q

Longer DNA =

A

Higher Tm

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5
Q

Tm depends on pH and

A

Ionic strength

HIGH SALT🍟 increase Tm

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Biochem Exam 3 (8 decks)

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