Therapeutic protein biontech

Question 1

I N S U L I N

Answer 1

• Has an isoelectric point of 5.3
• Can associate into hexameric complexes in the presence of 2+ metal ions
• Contain phenol as microbial agents

Question 2

R A P I D A C T I N G

Answer 2

• Insulin lispro

• Onset in 10-15 mins
• Inject prior to meal
• Lasts for 3-5 hours

Question 3

L O N G A C T I N G

Answer 3

• Insulin glargine
- Acidic
- Micropercipitates after subcut injection
- Crystals dissolve slowly
- Release after 4-5 hours and sustained for 24 hours
• Insulin detemir
- Covalent link to a fatty acid that enhances binding to albumin
- Released over 24 hours

Question 4

I N T E R M E D I A T E A C T I N G I N S U L I N

Answer 4

• Neutral protamine Hagedorn (NPH)
• Cocrystallisation of insulin with protamine for prolonged action
• Can be mixed with regular insulin in 30:70 ratio