Theme 2 modules 1 & 2

Question 1

transcription in prokaryotes

Answer 1

DNA is split and the template strand will be used to generate a complementary and antiparallel RNA molecule
This RNA molecule will have the same sequence as the non–template strand, but will have uracil instead of thymine

Question 2

what are the 3 steps of RNA transcription?

Answer 2

initiation, elongation, termination

Question 3

initiation

Answer 3

RNA polymerase binds to promoter region

Question 4

elongation

Answer 4

Will read the DNA and form an RNA strand complementary to the template strand

Question 5

termination

Answer 5

RNA polymerase will reach the termination region (at 3’ end of the gene) and will reform the double helix. This will release the RNA transcript and the transcription complex

Question 6

RNA transcription

Answer 6

RNA polymerase will bind to the promoter regions on the DNA
Promoter regions is where RNA synthesis will begin, (upstream from the 5’ end)
RNA polymerase will begin transcription and move along the DNA in a 3’ to 5’ direction
This synthesize the RNA in a 5’ to 3’ end
RNA Polymerase will continue to transcribe along the DNA until a transcription until it reaches the terminator, in which the RNA is released
Then the RNA polymerase is disassembled

Question 7

sigma factors

Answer 7

a protein which can identify specific promoters which help with initiation of transcription in prokaryotes. Help with turning on and off genes