The Microscope

Question 1

What are the two systems of lenses in a light microscope?

Answer 1

The ocular/eyepiece lens

The objective lens

Question 2

List some different microscopes.

Answer 2

Inverted phase contract microscope

A fluorescent microscope

Electron microscope

Question 3

What are the two different types of electron microscopes?

Answer 3

Transmission electron microscopes

Scanning electron microscopes

Question 4

How does a fluorescent microscope work?

Answer 4

A fluorescent microscope uses filters within the microscope to filter the light on fixed specimens

Question 5

How do electron microscopes work?

Answer 5

Electron microscopes use beams of electrons and magnetic lenses

Question 6

What does a transmission electron microscope do?

Answer 6

Used to view the inside of a biological specimen

Question 7

What does a scanning electron microscope do?

Answer 7

Used to view the surface topography of a specimen

Question 8

List the eight different parts of a microscope.

Answer 8

x10 ocular lens

Objective lenses

Mechanical stage with specimen holder/clips and Vernier scale

Condenser lens

Iris diaphragm

Light source

Course focus knob

Fine focus knob

Question 9

What are fluorochromes?

Answer 9

Fluorescent dyes that attach to visible or sub-visible structures, highly specific

Question 10

List three fluorochromes.

Answer 10

DAPI

Acridine orange

Rhodamine

Question 11

What is immunofluorescence?

Answer 11

Using antibodies to which a fluorescent marker has been attached

Question 12

What is electron microscopy?

Answer 12

The use of beams of electrons and magnetic coils instead of white light and glass lenses in the light microscope